Lipase A Antibody [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-54155CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP1-54155AF350

Alexa Fluor 405

NBP1-54155AF405

Alexa Fluor 488

NBP1-54155AF488

Alexa Fluor 532

NBP1-54155AF532

Alexa Fluor 594

NBP1-54155AF594

Alexa Fluor 647

NBP1-54155AF647

Alexa Fluor 700

NBP1-54155AF700

Alexa Fluor 750

NBP1-54155AF750

Allophycocyanin

NBP1-54155APC

Biotin

NBP1-54155B

DyLight 350

NBP1-54155UV

DyLight 405

NBP1-54155V

DyLight 488

NBP1-54155G

DyLight 550

NBP1-54155R

DyLight 594

NBP1-54155DL594

DyLight 650

NBP1-54155C

DyLight 680

NBP1-54155FR

DyLight 755

NBP1-54155IR

FITC

NBP1-54155F

HRP

NBP1-54155H

Janelia Fluor 525

NBP1-54155JF525

Janelia Fluor 549

NBP1-54155JF549

Janelia Fluor 585

NBP1-54155JF585

Janelia Fluor 635

NBP1-54155JF635

Janelia Fluor 646

NBP1-54155JF646

Janelia Fluor 669

NBP1-54155JF669

mFluor Violet 450 SE

NBP1-54155MFV450

mFluor Violet 500 SE

NBP1-54155MFV500

mFluor Violet 610 SE

NBP1-54155MFV610

PE

NBP1-54155PE

PerCP

NBP1-54155PCP

Forumulation

Catalog #

BSA Free

NBP1-54155

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

CoraFluor 1

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all LIPA Antibodies »

Product Summary for Lipase A Antibody [CoraFluor™ 1]

Immunogen

Partial recombinant protein made to an internal region of the human Lipase A protein (within residues 150-300). [Swiss-Prot P38571]

Reactivity Notes

Immunogen sequence has 82% identity to pig and 73% identity to rat and cow.

Localization

Lysosome.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for Lipase A Antibody [CoraFluor™ 1]

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: LIPA

Lipase A, also known as LIPA or Lysosomal acid lipase/cholesteryl ester hydrolase, has a 399 amino acid long isoform that is 45 kDa and a short 343 amino acid isoform that is 39 kDa; lysosome located; is very important for the intracellular hydrolysis of cholesteryl esters and triglycerides that have been internalized via receptor-mediated endocytosis of lipoprotein particles, and also in mediating the effect of LDL (low density lipoprotein) uptake on suppression of hydroxymethylglutaryl-CoA reductase and activation of endogenous cellular cholesteryl ester formation. This protein is currently being studied for involvement in the following diseases and disorders: cholesteryl ester storage disease, wolman disease, cholesterol, cholesterol ester storage disease, lysosomal storage disease, coronary heart disease, hepatitis c, splenomegaly, hepatitis b, hepatitis, hyperlipidemia, hypercholesterolemia, tuberculosis, obesity atherosclerosis, alzheimer's disease, neisseria meningitides, lipid storage disease, lipodystrophy, pandas, wolman disease, and blepharitis. The LIPA protein has been shown to interact with HIST1H4A, HIST1H4B, HIST1H4C, SOAT1, and HIST1H4E in cholesterol and sphingolipids transport / distribution to the intracellular membrane compartments (normal and CF), steroid biosynthesis, and lysosome pathways.

Long Name

Lipase A

Alternate Names

CESD, Lipase A

Gene Symbol

LIPA

Additional LIPA Products

Product Documents for Lipase A Antibody [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Lipase A Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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