Key Product Details

Species Reactivity

Porcine

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 28

Format

BSA Free

Concentration

1.0 mg/ml

View all Lipocalin-2/NGAL Antibodies »

Product Specifications

Immunogen

Recombinant pig NGAL

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Lipocalin-2/NGAL Antibody (28) - BSA Free

Immunohistochemistry: Lipocalin-2/NGAL Antibody (28) - BSA Free [NBP1-30006]

Immunohistochemistry: Lipocalin-2/NGAL Antibody (28) - BSA Free [NBP1-30006]

Immunohistochemistry: Lipocalin-2/NGAL Antibody (28) - BSA Free [NBP1-30006] - NGAL expression in the ischemic injured pig kidney; detected with anti-pig NGAL antibody (antibody dilution 1:25).
Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody (28) - BSA Free [NBP1-30006]

Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody (28) - BSA Free [NBP1-30006]

Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody (28) - BSA Free [NBP1-30006] - Positive staining of renal tubular cell cytoplasm, using mouse monoclonal antibody against pig NGAL. Courtesy of Oscar McCook, University of Ulm.
ELISA: Lipocalin-2/NGAL Antibody (28) - BSA Free [NBP1-30006]

ELISA: Lipocalin-2/NGAL Antibody (28) - BSA Free [NBP1-30006]

ELISA: Lipocalin-2/NGAL Antibody (28) - BSA Free [NBP1-30006] - The figure illustrates the calibration curve of a sandwich assay with NBP1-30005 as the capture antibody and NBP1-30006B as the biotinylated detection antibody.

Applications for Lipocalin-2/NGAL Antibody (28) - BSA Free

Application
Recommended Usage

ELISA

1:40000

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP1-30006 binds free pig NGAL in solution. NBP1-30006 (as biotinylated detection antibody) forms a sandwich ELISA pair with NBP1-30005 (as capture antibody) for measuring porcine NGAL. WB: NBP1-30006 can be used in Western blotting. IHC: NBP1-30006 detects NGAL in paraffin-embedded sections in the cytoplasm of renal tubular cell from pig.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Lipocalin-2/NGAL

Lipocalin 2 is a member of the lipocalin family which encompass more than 25 members (including proteins like retinol binding protein, a1 microglobulin, b lactoglobulin, apolipoprotein D and odorant binding protein). The members of the lipocalin family are characterized by their ability to bind small lipophilic substances in their hydrophobic core, and thereby serve as transporters of substances. Pig NGAL may, by analogy with its homologues in other mammalian species, be released from activated neutrophils in inflammation or infection, from certain epithelial cancers, and more dramatically, from renal tubular cells in response to ischemic or nephrotoxic injury.

Long Name

Neutrophil Gelatinase-associated Lipocalin

Alternate Names

24p3, LCN2, Lipocalin2, MSFI, NGAL, Oncogene 24p3, p25, Siderocalin, Uterocalin

Gene Symbol

LCN2

Additional Lipocalin-2/NGAL Products

Product Documents for Lipocalin-2/NGAL Antibody (28) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Lipocalin-2/NGAL Antibody (28) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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