Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 kappa Clone # 29

Format

BSA Free

Concentration

1.0 mg/ml

View all Lipocalin-2/NGAL Antibodies »

Product Specifications

Immunogen

Recombinant mouse NGAL

Clonality

Monoclonal

Host

Rat

Isotype

IgG1 kappa

Scientific Data Images for Lipocalin-2/NGAL Antibody (29) - BSA Free

Immunohistochemistry: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183]

Immunohistochemistry: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183]

Immunohistochemistry: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183] - NGAL expression 2 days after the introduction of ischemic-reperfusion injured mouse kidney;detected with anti-mouse NGAL antibody at dilution of 1:1000. Image and data supplied by Prof. Gueler, Medizinische Hochschule Hannover.
Immunohistochemistry: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183]

Immunohistochemistry: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183]

Immunohistochemistry: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183] - NGAL expression in the ischemia-reperfusion injured mouse kidney; detected with anti-mouse NGAL antibody at dilution of 1:1000. Image and data supplied by Prof. Gueler, Medizinische Hochschule Hannover.
ELISA: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183]

ELISA: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183]

ELISA: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183] - The figure illustrates the calibration curve of a sandwich assay with NBP2-21746 as the capture antibody and NBP1-05183B as the biotinylated detection antibody.

Applications for Lipocalin-2/NGAL Antibody (29) - BSA Free

Application
Recommended Usage

ELISA

1:4000

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP1-05183 binds free mouse NGAL in solution. NBP1-05183 forms an antibody pair for sandwich ELISA with NBP2-21746. It is recommended to use NBP2-21746 as a capture antibody and biotinylated NBP1-05183 as a detection antibody. WB: NBP1-05183 can be used in Western blotting. IHC: NBP1-05183 detects NGAL in paraffin-embedded sections in ischemia-reperfusion injured kidney cells from mouse (dilution 1:1000). Microwave pretreatment using Citrat buffer pH 6, is recommended.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Lipocalin-2/NGAL

Mouse neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, 24p3, SIP24, uterocalin and siderocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. 24p3 was observed to be dramatically upregulated in kidney cell cultures infected with SV40 (1) and subsequently identified as a lipocalin (2). Unlike human NGAL, it is an acute phase protein upregulated in the liver (3), but like its mammalian homologues it is upregulated and secreted by the kidney in response to ischemic or nephrotoxic injury (4). It is also upregulated in the involuting uterus and mammary gland (5).

Long Name

Neutrophil Gelatinase-associated Lipocalin

Alternate Names

24p3, LCN2, Lipocalin2, MSFI, NGAL, Oncogene 24p3, p25, Siderocalin, Uterocalin

Gene Symbol

LCN2

Additional Lipocalin-2/NGAL Products

Product Documents for Lipocalin-2/NGAL Antibody (29) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Lipocalin-2/NGAL Antibody (29) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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