Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 32

Format

BSA Free

Concentration

1.0 mg/ml

View all Lipocalin-2/NGAL Antibodies »

Product Specifications

Immunogen

Recombinant rat NGAL precursor

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Lipocalin-2/NGAL Antibody (32) - BSA Free

ELISA: Lipocalin-2/NGAL Antibody (32) - BSA Free [NBP1-05182]

ELISA: Lipocalin-2/NGAL Antibody (32) - BSA Free [NBP1-05182]

ELISA: Lipocalin-2/NGAL Antibody (32) - BSA Free [NBP1-05182] - The figure illustrates the calibration curve of a sandwich assay with NBP1-05182 as the capture antibody and NBP1-05182B as the biotinylated detection antibody.

Applications for Lipocalin-2/NGAL Antibody (32) - BSA Free

Application
Recommended Usage

ELISA

1:4000

Immunohistochemistry

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP1-05182 binds free rat NGAL in solution. WB: NBP1-05182 can be used in Western blotting. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 27136530). ICC/IF reactivity reported in scientific literature (PMID: 27136530).

Reviewed Applications

Read 1 review rated 3 using NBP1-05182 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Lipocalin-2/NGAL

Rat NGAL, so called because it is the ortholog of human neutrophil gelatinase-associated lipocalin (NGAL), is also called lipocalin 2, 25-kDa alpha 2-microglobulin-related protein or neu-related lipocalin. It is expressed by neutrophils and various epithelial cells, including cells of the renal proximal tubules. It is involved in physiological processes such as the binding of siderophore iron and tissue growth and regeneration. It is upregulated in a variety of pathological situations including infection, inflammation, ischemia-reperfusion and in certain adenocarcinomas.

Long Name

Neutrophil Gelatinase-associated Lipocalin

Alternate Names

24p3, LCN2, Lipocalin2, MSFI, NGAL, Oncogene 24p3, p25, Siderocalin, Uterocalin

Gene Symbol

LCN2

Additional Lipocalin-2/NGAL Products

Product Documents for Lipocalin-2/NGAL Antibody (32) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Lipocalin-2/NGAL Antibody (32) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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