Lipocalin-2/NGAL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1503

Novus Biologicals, part of Bio-Techne
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NB100-1503
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Lipocalin-2/NGAL Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-AILREDKDPQKMY corresponding to internal region according to NP_005555.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Lipocalin-2/NGAL Antibody

Western Blot: Lipocalin-2/NGAL Antibody [NB100-1503]

Western Blot: Lipocalin-2/NGAL Antibody [NB100-1503]

Western Blot: Lipocalin-2/NGAL Antibody [NB100-1503] - (0.03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody [NB100-1503]

Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody [NB100-1503]

Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody [NB100-1503] - Analysis of LCN2 in human xenograft tumor. Image courtesy of product review submitted by Yi-Ling Huang.
Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody [NB100-1503]

Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody [NB100-1503]

Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody [NB100-1503] - (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Lipocalin-2/NGAL Antibody

Application
Recommended Usage

Immunohistochemistry

2 - 3 ug/ml

Immunohistochemistry-Paraffin

2 - 3 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.01 - 0.03 ug/ml
Application Notes
WB: Approx. 22 kDa band observed in human kidney lysates (calculated MW of 22.6 kDa band according to NP_005555.2). IHC-P: Human spleen shows staining of cytoplasm in mostly binucleated cells, and of nucleoli in some of the non-dividing cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Lipocalin-2/NGAL

Lipocalin 2 is a member of the lipocalin family which encompass more than 25 members (including proteins like retinol binding protein, a1 microglobulin, b lactoglobulin, apolipoprotein D, odorant binding protein). The members of the lipocalin family are characterized by their ability to bind small lipophilic substances in their hydrophobic core, and thereby serve as transporters of substances.

Long Name

Neutrophil Gelatinase-associated Lipocalin

Alternate Names

24p3, LCN2, Lipocalin2, MSFI, NGAL, Oncogene 24p3, p25, Siderocalin, Uterocalin

Entrez Gene IDs

3934 (Human)

Gene Symbol

LCN2

UniProt

NP_005555.2. (Human)

Additional Lipocalin-2/NGAL Products

Product Documents for Lipocalin-2/NGAL Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Lipocalin-2/NGAL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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