LITAF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83473

Novus Biologicals, part of Bio-Techne
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NBP1-83473
NBP1-83473-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all LITAF Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: APPSYEETVAVNSYYPTPPAPMPGPTTGLVTGPDGKGMNPPSYYTQPAPIPNNNPITVQTVYVQHPITFLDRPIQMCCPSCNKMIVSQLSYNAGALTWLSCGSLCLLGCIAGCCFIPFCVDALQDVDHYCPNC

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (89%). Reactivity reported in scientific literature (PMID: 23576546)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LITAF Antibody

Western Blot: LITAF Antibody [NBP1-83473]

Western Blot: LITAF Antibody [NBP1-83473]

Western Blot: LITAF Antibody [NBP1-83473] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: LITAF Antibody [NBP1-83473]

Immunocytochemistry/ Immunofluorescence: LITAF Antibody [NBP1-83473]

Immunocytochemistry/Immunofluorescence: LITAF Antibody [NBP1-83473] - Staining of human cell line A-431 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: LITAF Antibody [NBP1-83473]

Immunohistochemistry-Paraffin: LITAF Antibody [NBP1-83473]

Immunohistochemistry-Paraffin: LITAF Antibody [NBP1-83473] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.

Applications for LITAF Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LITAF

Lipopolysaccharide is a potent stimulator of monocytes and macrophages, causing secretion of tumor necrosis factor-alpha (TNF-alpha) and other inflammatory mediators. This gene encodes lipopolysaccharide-induced TNF-alpha factor, which is a DNA-binding protein and can mediate the TNF-alpha expression by direct binding to the promoter region of the TNF-alpha gene. The transcription of this gene is induced by tumor suppresor p53 and has been implicated in the p53-induced apoptotic pathway. Mutations in this gene cause Charcot-Marie-Tooth disease type 1C (CMT1C) and may be involved in the carcinogenesis of extramammary Paget's disease (EMPD). Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Long Name

Lipopolysaccharide-induced TNF Factor

Alternate Names

PIG7, SIMPLE, TP53I7

Gene Symbol

LITAF

Additional LITAF Products

Product Documents for LITAF Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for LITAF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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