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LMO2 Antibody (OTI1G10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45787

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1G10

Concentration

1 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human LMO2(NP_005565) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

18.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LMO2 Antibody (OTI1G10)

Western Blot: LMO2 Antibody (OTI1G10) [NBP2-45787]

Western Blot: LMO2 Antibody (OTI1G10) [NBP2-45787]

Western Blot: LMO2 Antibody (1G10) [NBP2-45787] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMO2.
Immunohistochemistry: LMO2 Antibody (OTI1G10) [NBP2-45787]

Immunohistochemistry: LMO2 Antibody (OTI1G10) [NBP2-45787]

Immunohistochemistry: LMO2 Antibody (1G10) [NBP2-45787] - Analysis of Human prostate tissue (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: LMO2 Antibody (OTI1G10) [NBP2-45787]

Immunohistochemistry: LMO2 Antibody (OTI1G10) [NBP2-45787]

Immunohistochemistry: LMO2 Antibody (1G10) [NBP2-45787] - Analysis of Human bladder. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Applications for LMO2 Antibody (OTI1G10)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:4000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LMO2

LMO2 is a highly conserved oncoprotein that is expressed in human normal germinal-center B cells and in B-cell lymphomas. LMO2 also has a crucial role in hematopoietic development by being necessary for yolk sac erythropoiesis. LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number of T-cell acute lymphoblastic leukemia-specific translocations occur.

LMO2 antibodies are useful tools for erythropoiesis research and certian cancer studies.

Long Name

LIM Domain Only 2

Alternate Names

RBTN2, RBTNL1, RHOM2, TTG2

Gene Symbol

LMO2

Additional LMO2 Products

Product Documents for LMO2 Antibody (OTI1G10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LMO2 Antibody (OTI1G10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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