LMO2 Antibody (rLMO2/1971)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07253

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rLMO2/1971

Concentration

0.2 mg/ml

View all LMO2 Antibodies »

Product Summary for LMO2 Antibody (rLMO2/1971)

Immunogen

Recombinant human LMO2 protein fragment (around aa 23-140) (exact sequence is proprietary) (Uniprot: P25791)

Localization

Nucleus, cytoplasm and Cell surface

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08285)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for LMO2 Antibody (rLMO2/1971)

Western Blot: LMO2 Antibody (rLMO2/1971) [NBP3-07253]

Western Blot: LMO2 Antibody (rLMO2/1971) [NBP3-07253]

Western Blot: LMO2 Antibody (rLMO2/1971) [NBP3-07253] - Western blot analysis of THP1 cell lysate using LMO2 antibody (rLMO2/1971).
Immunohistochemistry-Paraffin: LMO2 Antibody (rLMO2/1971) [NBP3-07253]

Immunohistochemistry-Paraffin: LMO2 Antibody (rLMO2/1971) [NBP3-07253]

Immunohistochemistry-Paraffin: LMO2 Antibody (rLMO2/1971) [NBP3-07253] - Formalin-fixed, paraffin-embedded human pancreas stained with LMO2 Mouse Monoclonal Antibody (rLMO2/1971).
Immunohistochemistry-Paraffin: LMO2 Antibody (rLMO2/1971) [NBP3-07253]

Immunohistochemistry-Paraffin: LMO2 Antibody (rLMO2/1971) [NBP3-07253]

Immunohistochemistry-Paraffin: LMO2 Antibody (rLMO2/1971) [NBP3-07253] - Formalin-fixed, paraffin-embedded human pancreas stained with LMO2 Mouse Monoclonal Antibody (rLMO2/1971).

Applications for LMO2 Antibody (rLMO2/1971)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: LMO2

LMO2 is a highly conserved oncoprotein that is expressed in human normal germinal-center B cells and in B-cell lymphomas. LMO2 also has a crucial role in hematopoietic development by being necessary for yolk sac erythropoiesis. LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number of T-cell acute lymphoblastic leukemia-specific translocations occur.

LMO2 antibodies are useful tools for erythropoiesis research and certian cancer studies.

Long Name

LIM Domain Only 2

Alternate Names

RBTN2, RBTNL1, RHOM2, TTG2

Gene Symbol

LMO2

UniProt

P25791

Additional LMO2 Products

Product Documents for LMO2 Antibody (rLMO2/1971)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LMO2 Antibody (rLMO2/1971)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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