LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71261

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1D1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all LMP2/PSMB9 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 21-219 of human PSMB9(NP_002791) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

23.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free

Western Blot: LMP2/PSMB9 Antibody (OTI1D1)Azide and BSA Free [NBP2-71261]

Western Blot: LMP2/PSMB9 Antibody (OTI1D1)Azide and BSA Free [NBP2-71261]

Western Blot: LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71261] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 20S LMP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Pr
Immunocytochemistry/ Immunofluorescence: LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71261]

Immunocytochemistry/ Immunofluorescence: LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71261]

Immunocytochemistry/Immunofluorescence: LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71261] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Proteasome 20S LMP2.
Immunohistochemistry: LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71261]

Immunohistochemistry: LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71261]

Immunohistochemistry: LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71261] - Staining of paraffin-embedded Human Kidney tissue using anti-Proteasome 20S LMP2 mouse monoclonal antibody.
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Applications for LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LMP2/PSMB9

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Other proteins are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. The PA28/11S REG Activator protein complex functions as a proteolytic activator. LMP2 is a catalytic subunit of the 20S proteasome and, upon interferon gamma-induction, replaces the delta subunit. LMP2 alters the specificity of the 20S proteasome and is critical for the production of MHC class I ligands, production of T-lymphocytes, and is suggested to increase the efficiency of antigen presentation of the immune response. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis (CF transmembrane regulator), Angelman's syndrome (E6-AP), and Liddle syndrome (endothelial sodium channels).

Long Name

Proteasome (Prosome, Macropain) Subunit, beta Type, 9

Alternate Names

Proteasome subunit beta-1i, PSMB9, RING12

Gene Symbol

PSMB9

Additional LMP2/PSMB9 Products

Product Documents for LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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