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Loricrin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33610

Novus Biologicals, part of Bio-Techne
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NBP1-33610

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rhesus Macaque (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Loricrin. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (87%).

Localization

Cytoplasm, Nucleus, nucleoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Loricrin Antibody

Immunohistochemistry: Loricrin Antibody [NBP1-33610]

Immunohistochemistry: Loricrin Antibody [NBP1-33610]

Immunohistochemistry: Loricrin Antibody [NBP1-33610] - Prominent differences in the expressions of innate immune and barrier molecules between sebaceous gland poor (SGR) and sebaceous gland rich (SGP) skin regions. Representative images for immunostaining and quantification of epidermal levels of LOR in SGP and SGR skin sections. Images of negative control stainings are shown in the bottom left corner of SGP immunostainings. Size bars=100um. The graphs show the mean +/- SEM of measured protein levels (*p<0.05; **p<0.01; ***p<0.001, as determined by Mann-Whitney U-test). Image collected and cropped by CiteAb from the following publication (frontiersin.org/articles/10.3389/fimmu.2018.00424/full), licensed under a CC-BY license.
Western Blot: Loricrin Antibody [NBP1-33610]

Western Blot: Loricrin Antibody [NBP1-33610]

Western Blot: Loricrin Antibody [NBP1-33610] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Loricrin antibody [N1], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Loricrin Antibody [NBP1-33610]

Immunocytochemistry/ Immunofluorescence: Loricrin Antibody [NBP1-33610]

Immunocytochemistry/Immunofluorescence: Loricrin Antibody [NBP1-33610] - Analysis of methanol-fixed Hep3B, using antibody at 1:500 dilution.

Applications for Loricrin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Simple Western

Validated from a verified customer review.

Western Blot

1:500-1:3000

Reviewed Applications

Read 3 reviews rated 4.3 using NBP1-33610 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Loricrin

This gene encodes loricrin, a major protein component of the cornified cell envelope found in terminally differentiated epidermal cells. Mutations in this gene are associated with Vohwinkel's syndrome and progressive symmetric erythrokeratoderma, both inherited skin diseases. [provided by RefSeq]

Alternate Names

loricrin, LRN, MGC111513

Gene Symbol

LORICRIN

Additional Loricrin Products

Product Documents for Loricrin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Loricrin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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