LRH-1/NR5A2 Antibody (PCRP-NR5A2-1B8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26786

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-NR5A2-1B8

Concentration

0.2 mg/ml

View all LRH-1/NR5A2 Antibodies »

Product Specifications

Immunogen

Recombinant human LRH-1/NR5A2 protein

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for LRH-1/NR5A2 Antibody (PCRP-NR5A2-1B8)

LRH-1/NR5A2 Antibody (PCRP-NR5A2-1B8)

Flow Cytometry: LRH-1/NR5A2 Antibody (PCRP-NR5A2-1B8) [NBP3-26786 ] -

Flow Cytometric Analysis of PFA-fixed HeLa cells. LRH-1/NR5A2 Antibody (PCRP-NR5A2-1B8) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
LRH-1/NR5A2 Antibody (PCRP-NR5A2-1B8)

Protein Array: LRH-1/NR5A2 Antibody (PCRP-NR5A2-1B8) [NBP3-26786 ] -

Analysis of Protein Array containing more than 19,000 full-length human proteins using LRH-1/NR5A2 Antibody (PCRP-NR5A2-1B8).
LRH-1/NR5A2 Antibody (PCRP-NR5A2-1B8)

Immunohistochemistry-Paraffin: LRH-1/NR5A2 Antibody (PCRP-NR5A2-1B8) [NBP3-26786 ] -

Formalin-fixed, paraffin-embedded human prostate cancer stained with LRH-1/NR5A2 Antibody (PCRP-NR5A2-1B8). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications for LRH-1/NR5A2 Antibody (PCRP-NR5A2-1B8)

Application
Recommended Usage

Flow Cytometry

1-2ug/million cells

Immunohistochemistry-Paraffin

1-2ug/ml for 30 minutes at RT
Application Notes
Immunohistochemistry-Paraffin 1-2ug/ml for 30 minutes at RT. Optimal dilution for a specific application should be determined.. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: LRH-1/NR5A2

Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentialtion of epithelial, neuronal, glial, and other types of cells.

Long Name

Liver Receptor Homolog 1/Nuclear Receptor Subfamily 5, Group A, Member 2

Alternate Names

B1F2, FTZ-F1 beta, LRH1, NR5A2

Gene Symbol

NR5A2

Additional LRH-1/NR5A2 Products

Product Documents for LRH-1/NR5A2 Antibody (PCRP-NR5A2-1B8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LRH-1/NR5A2 Antibody (PCRP-NR5A2-1B8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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