LRP-1 Antibody (A2Mr alpha-2)

Immunohistochemistry: LRP-1 Antibody (A2Mr alpha-2) - BSA Free [NB100-64808]

Immunohistochemistry: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2 followed by the Histar detection system. Medium power.

Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) - BSA Free [NB100-64808]

Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2, red in A and Mouse anti Human CD21, clone LB21, green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power.

Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) - BSA Free [NB100-64808]

Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2, red in A and Mouse anti Human CD21, clone LB21, green in B. C is the merged image with nuclei counterstained blue using DAPI. Medium power.

Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) - BSA Free [NB100-64808]

Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2, red in A and Mouse anti Human CD21, clone LB21, green in B. C is the merged image with nuclei counterstained blue using DAPI. High power.

Immunocytochemistry/ Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] -

Immunocytochemistry/ Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Reduced EHD1 recruitment to endosomes upon EHD4 knock-down.A-F, Representative micrographs & insets depicting EHD1-GFP recruitment to endosomes in untreated (A & inset in D), mock-treated (B & inset in E), & EHD4 knock-down (C & inset in F) cells. CRISPR/Cas9 gene-edited NIH3T3 cells expressing endogenous levels of EHD1 with GFP fused to the C-terminus (EHD1-GFP) were either untreated, mock-treated with transfection reagent, or transfected with EHD4 siRNA for 72 h. Cells were then incubated with anti-LRP1 antibody (30 min on ice, 30 min at 37°C), fixed, & imaged via confocal microscopy. G, Immunoblot showing reduced EHD4 (but not EHD1-GFP) expression in EHD1-GFP cells, with actin used as a loading control. The nitrocellulose filter paper was then stripped & immunoblotted with anti-GFP to show EHD1-GFP expression upon EHD4 loss. H, Graph depicting the mean count of EHD1-labeled endosomes in untreated, mock-treated & EHD4-depleted cells. Individual experiments were performed 3 times. Error bars denote standard deviation & p-values were determined by one-way ANOVA for individual experiments using a post-hoc Tukey HSD calculator to determine significance. A consensus p-value was then derived as described in the Materials & methods to assess significant differences between samples from the 3 experiments. Micrographs are representative orthogonal projects from three independent experiments, with 10 sets of z-stacks collected for each treatment per experiment Bar, 10 μm. n.s., not significant (consensus p > 0.5). Consensus p-values from Tukey HSD: *p < 0.00001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32966336), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] -

Immunocytochemistry/ Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Increased sorting endosome size & decreased EHD1 recruitment upon Rabenosyn-5 knock-down.A-L, Representative micrographs & insets depicting EEA1-labeled endosomes & EHD1-GFP in mock-treated & Rabenosyn-5 knock-down cells. CRISPR/Cas9 gene-edited NIH3T3 EHD1-GFP cells were either mock-treated with transfection reagent (A-F) or treated with Rabenosyn-5 siRNA (G-L) for 72 h. Cells were then incubated with anti-LRP1 antibody (30 min on ice, 30 min at 37°C), fixed & immunostained using anti-EEA1, & imaged by confocal microscopy. M, Immunoblot showing reduced Rabenosyn-5 expression in EHD1-GFP NIH3T3 cells. N, Graph depicting mean endosome size of mock-treated & Rabenosyn-5 knock-down cells. O, Graph depicting EHD1 recruitment to endosomes in mock-treated & Rabenosyn-5 knock-down cells. Error bars denote standard deviation & p-values were determined by independent two-tailed t-test, with significance derived from consensus p-values from the 3 experiments. Micrographs are representative orthogonal projections from three independent experiments, with 10 sets of z-stacks collected for each treatment per experiment. Bar, 10 μm. **p < 0.00001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32966336), licensed under a CC-BY license. Not internally tested by Novus Biologicals.