LRRK2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-55289

Novus Biologicals, part of Bio-Techne
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NB110-55289
NB110-55289SS
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Key Product Details

Species Reactivity

Validated:

Mouse, Human (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.09 mg/ml

View all LRRK2 Antibodies »

Product Summary for LRRK2 Antibody

Immunogen

A synthetic peptide made to an internal region of the mouse LRRK2 protein sequence (between residues 900-1000).

Reactivity Notes

Does not react with Human.

Localization

Mitochondrial outer membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

285 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LRRK2 Antibody

Western Blot: LRRK2 Antibody [NB110-55289]

Western Blot: LRRK2 Antibody [NB110-55289]

Western Blot: LRRK2 Antibody [NB110-55289] - Detection of murine LRRK2 in mouse brain lysate.
Immunohistochemistry: LRRK2 Antibody [NB110-55289]

Immunohistochemistry: LRRK2 Antibody [NB110-55289]

Immunohistochemistry: LRRK2 Antibody [NB110-55289] - Staining of neurons and glia. Mouse Brain, 40X.

Applications for LRRK2 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

2 ug/ml
Application Notes
In Western blot, a band is seen at ~284 kDa. There is a band at ~171 and 117 kDa, as well as several lower molecular weight, non-specific bands.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.1% Sodium Azide

Concentration

1.09 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: LRRK2

LRRK2 (leucine-rich repeat serine/threonine-protein kinase 2, also called PARK8 or Dardarin) is a homodimeric multifunctional protein that belongs to TKL serine threonine protein kinases family and LRRK2 immunoreactivity has been documented in neurofibrillary tangles in Alzheimer's disease as well as in PDCG (parkinsonism-dementia complex of Guam). Highly expressed in tissues such as liver, heart, brain etc., LRRK2 localizes to cellular cytoplasm and also associates with membrane structures including mitochondrial outer membrane. LRRK interacts with PARK2, PRDX3 and TPCN2, and phosphorylate proteins which are implicated in Parkinson disease. LRRK2 is a cytoplasmic kinase with autophosphorylation capability as well as GTPase activity. Its interaction with microtubules suggests that LRRK2-induced neurodegeneration might be partly mediated via inhibition of microtubule dynamics, and LRRK2 -mitochondria interactiosn suggests its implication in pathways eliciting ROS mediated damage. LRRK2 has been shown exert positive regulation in autophagy via calcium-sensitive activation of CaMKK/AMPK signaling pathway through activation of NAADP receptors, increase of lysosomal pH as well as calcium release.

Long Name

Leucine-rich Repeat Ser/Thr Protein Kinase 2

Alternate Names

AURA17, Dardarin, PARK8, ROCO2

Gene Symbol

LRRK2

Additional LRRK2 Products

Product Documents for LRRK2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LRRK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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