LSD1 Antibody (2E7)

Novus Biologicals, part of Bio-Techne | Catalog # H00023028-M04

Novus Biologicals, part of Bio-Techne
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H00023028-M04
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Antibodies »

Product Specifications

Immunogen

AOF2 (NP_055828, 753 a.a. ~ 851 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ADPWARGSYSYVAAGSSGNDYDLMAQPITPGPSIPGAPQPIPRLFFAGEHTIRNYPATVHGALLSGLREAGRIADQFLGAMYTLPRQATPGVPAQQSPS

Specificity

AOF2 (2E7)

Marker

Nucleus Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for LSD1 Antibody (2E7)

Immunocytochemistry/ Immunofluorescence: LSD1 Antibody (2E7) [H00023028-M04]

Immunocytochemistry/ Immunofluorescence: LSD1 Antibody (2E7) [H00023028-M04]

Immunocytochemistry/Immunofluorescence: LSD1 Antibody (2E7) [H00023028-M04] - Analysis of monoclonal antibody to AOF2 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: LSD1 Antibody (2E7) [H00023028-M04]

Immunohistochemistry-Paraffin: LSD1 Antibody (2E7) [H00023028-M04]

Immunohistochemistry-Paraffin: LSD1 Antibody (2E7) [H00023028-M04] - Analysis of monoclonal antibody to AOF2 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 1 ug/ml
ELISA: LSD1 Antibody (2E7) [H00023028-M04]

ELISA: LSD1 Antibody (2E7) [H00023028-M04]

ELISA: LSD1 Antibody (2E7) [H00023028-M04] - Detection limit for recombinant GST tagged AOF2 is approximately 0.3ng/ml as a capture antibody.

Applications for LSD1 Antibody (2E7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 1A/KDM1A/LSD1

This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternate splicing has been observed; however, not all transcript variants have been fully characterized.

Alternate Names

AOF2, BHC110, LSD1, Lysine (K)specific Demethylase 1A

Entrez Gene IDs

23028 (Human)

Gene Symbol

KDM1A

OMIM

609132 (Human)

UniProt

NP_055828 (Human)

Additional Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Products

Product Documents for LSD1 Antibody (2E7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for LSD1 Antibody (2E7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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