Key Product Details

Species Reactivity

Human

Applications

Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 50 and 100 of human FAD-binding protein BRAF35-HDAC complex 110 kDa using the numbering given in TrEMBL entry O60341 (GeneID 23028).

Specificity

NB100-536 is specific for human BHC110-KIAA0601 protein.

Marker

Nucleus Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LSD1 Antibody - BSA Free

Western Blot: LSD1 Antibody [NB100-536]

Western Blot: LSD1 Antibody [NB100-536]

Western Blot: LSD1 Antibody [NB100-536] - Detection of human LSD1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-LSD1 antibody NB100-536 (lot NB100-536-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. LSD1 was also immunoprecipitated by a previous lot (lot NB100-536-1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Immunohistochemistry-Paraffin: LSD1 Antibody [NB100-536]

Immunohistochemistry-Paraffin: LSD1 Antibody [NB100-536]

Immunohistochemistry-Paraffin: LSD1 Antibody [NB100-536] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BHC110/LSD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: LSD1 Antibody [NB100-536]

Western Blot: LSD1 Antibody [NB100-536]

Western Blot: LSD1 Antibody [NB100-536] - Detection of Human BHC110/KIAA0601 by Western Blot. Samples: Nuclear extract (5 and 20 ug) from HeLa cells. Antibody: NB100-536 used at 0.01 ug/ml. Detection: Chemiluminescence with an exposure time of 15 minutes.

Applications for LSD1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

10 ug

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:5000-1:15000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Lysine (K)-specific Demethylase 1A/KDM1A/LSD1

This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Alternate Names

AOF2, BHC110, LSD1, Lysine (K)specific Demethylase 1A

Entrez Gene IDs

23028 (Human); 99982 (Mouse)

Gene Symbol

KDM1A

UniProt

O60341 (Human)

Additional Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Products

Product Documents for LSD1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for LSD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.