LSD1 Antibody

Western Blot: LSD1 Antibody [NBP3-18687]

Western Blot: LSD1 Antibody [NBP3-18687] - Figure 3 Immunoprecipitation and Western blot analysis using the antibody directed against LSD1 293T cells were transiently transfected with an expression vector for HA-tagged LSD1 (lanes 1) or with an empty vector (lanes 2). Figure 3A: Whole cell extracts were analysed by Western blot using the antibody against human LSD1 , diluted 1:1,000. Figure 3B. Whole cell extracts were immunoprecipitated with 2 ug of the antibody against LSD1. Immunoprecipitates were analysed by Western blot as described above.

Western Blot: LSD1 Antibody [NBP3-18687]

Western Blot: LSD1 Antibody [NBP3-18687] - Figure 2 Western blot analysis using the antibody directed against LSD1 Western blot was performed using respectively 150 ug (lane 1) or 50 ug (lane 2) nuclear extracts from HeLa cells and the antibody against LSD1 diluted 1:1,000. The location of the protein of interest is indicated on the left.

ELISA: LSD1 Antibody [NBP3-18687]

ELISA: LSD1 Antibody [NBP3-18687] - Figure 1 Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human LSD1 . The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,500.