LSM12 Antibody

Western Blot: LSM12 Antibody [NBP3-12550]

Western Blot: LSM12 Antibody [NBP3-12550] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa

Immunocytochemistry/ Immunofluorescence: LSM12 Antibody [NBP3-12550]

Immunocytochemistry/Immunofluorescence: LSM12 Antibody [NBP3-12550] - Staining of LSM12 in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human LSM12 polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Immunoprecipitation: LSM12 Antibody [NBP3-12550]

Immunoprecipitation: LSM12 Antibody [NBP3-12550] - Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-LSM12 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-LSM12 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size :22 kDa