Luciferase Antibody (HL2164) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25468
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Firefly
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2164
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragmemt of Luciferase
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Luciferase Antibody (HL2164) - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: Luciferase Antibody (HL2164) - Azide and BSA Free [NBP3-25468] -
Immunocytochemistry/Immunofluorescence: Luciferase Antibody (HL2164) - Azide and BSA Free [NBP3-25468] - Firefly Luciferase antibody [HL2164] detects Firefly Luciferase protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Firefly Luciferase stained by Firefly Luciferase antibody [HL2164] (NBP3-25468) diluted at 1:500. Blue: Fluoroshield with DAPI .
Western Blot: Luciferase Antibody (HL2164) - Azide and BSA Free [NBP3-25468] -
Western Blot: Luciferase Antibody (HL2164) - Azide and BSA Free [NBP3-25468] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Firefly Luciferase antibody [HL2164] (NBP3-25468) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Luciferase Antibody (HL2164) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Luciferase
The luciferase assay is fast and sensitive, differentiating itself from the CAT (chloramphenicol acetyltransferase) assay because it does not require a radioactive substrate.
References
1. Eun, H. (1996). Marker/Reporter enzymes. Enzymology Primer for Recombinant DNA Technology, 567-645. doi:10.1016/b978-012243740-3/50011-9
2. McNabb, D. S., Reed, R., & Marciniak, R. A. (2005). Dual luciferase assay system for rapid assessment of gene expression in Saccharomyces cerevisiae. Eukaryotic Cell, 4(9), 1539-1549. doi:10.1128/ec.4.9.1539-1549.2005
3. Fraga, H. (2008). Firefly luminescence: A historical perspective and recent developments. Photochemical & Photobiological Sciences, 7(2), 146-158. doi:10.1039/b719181b
4. Younes, A., Lukyanenko, Y. O., Lyashkov, A. E., Lakatta, E. G., & Sollott, S. J. (2011). A bioluminescence method for direct measurement of phosphodiesterase activity. Analytical Biochemistry, 417(1), 36-40. doi:10.1016/j.ab.2011.05.036
Alternate Names
LuC, luciferin 4 monooxygenase, Luciferin 4-monooxygenase
Additional Luciferase Products
Product Documents for Luciferase Antibody (HL2164) - Azide and BSA Free
Product Specific Notices for Luciferase Antibody (HL2164) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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