Luciferase Antibody [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1677F

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Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Unconjugated

NB100-1677

Alexa Fluor 350

NB100-1677AF350

Alexa Fluor 405

NB100-1677AF405

Alexa Fluor 488

NB100-1677AF488

Alexa Fluor 532

NB100-1677AF532

Alexa Fluor 594

NB100-1677AF594

Alexa Fluor 647

NB100-1677AF647

Alexa Fluor 700

NB100-1677AF700

Alexa Fluor 750

NB100-1677AF750

Allophycocyanin

NB100-1677APC

Biotin

NB100-1677B

Biotin

NB120-634

CoraFluor 1

NB100-1677CL1

DyLight 350

NB100-1677UV

DyLight 405

NB100-1677V

DyLight 488

NB100-1677G

DyLight 550

NB100-1677R

DyLight 594

NB100-1677DL594

DyLight 650

NB100-1677C

DyLight 680

NB100-1677FR

DyLight 755

NB100-1677IR

HRP

NB100-1676

HRP

NB100-1677H

Janelia Fluor 525

NB100-1677JF525

Janelia Fluor 549

NB100-1677JF549

Janelia Fluor 646

NB100-1677JF646

Janelia Fluor 669

NB100-1677JF669

PE

NB100-1677PE

PerCP

NB100-1677PCP

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Firefly

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Luciferase Antibodies »

Product Specifications

Immunogen

Luciferase [Photinus pyralis (Firefly)]

Reactivity Notes

No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.

Specificity

No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Luciferase Antibody [FITC]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Luciferase

Luciferase is a generic term for a group of oxidative enzymes used in bioluminescence. Firefly (Photinus pyralis) and bacterial luciferase enzymes are commonly used in assay systems such as cell viability assays, reporter gene assays, and for in vivo imaging. Bacterial luciferases are flavoenzymes composed of two subunits each encoded by the luxA and luxB genes, while the firefly luciferase is a single polypeptide specified by the luc gene (1). Firefly luciferase (theoretical molecular weight: 61 kDa) oxidizes the substrate luciferin to oxyluciferin in a bioluminescent reaction requiring Mg2+ and ATP (2,3). This reaction produces a flash of yellow-green light with an emission peak around 560nm that can be detected by a luminometer (3). Firefly luciferase has become one of the more widely used reporter proteins and is an excellent tool for the study of gene expression, given that the amount of light emitted is directly proportional to luciferase activity (4).

The luciferase assay is fast and sensitive, differentiating itself from the CAT (chloramphenicol acetyltransferase) assay because it does not require a radioactive substrate.

References

1. Eun, H. (1996). Marker/Reporter enzymes. Enzymology Primer for Recombinant DNA Technology, 567-645. doi:10.1016/b978-012243740-3/50011-9

2. McNabb, D. S., Reed, R., & Marciniak, R. A. (2005). Dual luciferase assay system for rapid assessment of gene expression in Saccharomyces cerevisiae. Eukaryotic Cell, 4(9), 1539-1549. doi:10.1128/ec.4.9.1539-1549.2005

3. Fraga, H. (2008). Firefly luminescence: A historical perspective and recent developments. Photochemical & Photobiological Sciences, 7(2), 146-158. doi:10.1039/b719181b

4. Younes, A., Lukyanenko, Y. O., Lyashkov, A. E., Lakatta, E. G., & Sollott, S. J. (2011). A bioluminescence method for direct measurement of phosphodiesterase activity. Analytical Biochemistry, 417(1), 36-40. doi:10.1016/j.ab.2011.05.036

Alternate Names

LuC, luciferin 4 monooxygenase, Luciferin 4-monooxygenase

Additional Luciferase Products

Product Documents for Luciferase Antibody [FITC]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Luciferase Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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