Ly49F Antibody (HBF-719) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28096

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # HBF-719

Format

Azide and BSA Free

Concentration

0.5 mg/ml

View all Ly49F Antibodies »

Product Specifications

Immunogen

CHO-K1 cells transfected with B6 allele of Ly49F gene

Specificity

B6 allele of the Ly49F receptor

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Ly49F Antibody (HBF-719) - Azide and BSA Free

Flow Cytometry: Ly49F Antibody (HBF-719) - Azide and BSA Free [NBP1-28096]

Flow Cytometry: Ly49F Antibody (HBF-719) - Azide and BSA Free [NBP1-28096]

Flow Cytometry: Ly49F Antibody (HBF-719) [NBP1-28096] - Analysis using the FITC conjugate of NBP1-28096. Multiple staining of C57BL/6 splenocytes.
Flow Cytometry: Ly49F Antibody (HBF-719) - Azide and BSA Free [NBP1-28096]

Flow Cytometry: Ly49F Antibody (HBF-719) - Azide and BSA Free [NBP1-28096]

Flow Cytometry: Ly49F Antibody (HBF-719) [NBP1-28096] - Analysis using the Biotin conjugate of NBP1-28096. Multiple staining of C57BL/6 splenocytes.

Applications for Ly49F Antibody (HBF-719) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

<= 1 ug/10^6 cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.1M BBS (pH 8.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Ly49F

The monoclonal antibody HBF-719 reacts with the B6 allele of Ly49F, a member of the lectin-like homodimeric Ly49 family of cell surface receptors.1 Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated. (2) These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells. The HBF-719 antibody stains approx. 11% of NK1.1+CD3- splenocytes, and 3.5% (2.5 month old) to 10.0% (11 month old) of CD8+ spleen cells from C57BL/6 mice. (1)

Alternate Names

Killer cell lectin like receptor 6, Klra6, Ly49F antigen, Lymphocyte antigen 49F, T cell surface glycoprotein Ly 49F

Gene Symbol

Klra6

Additional Ly49F Products

Product Documents for Ly49F Antibody (HBF-719) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Ly49F Antibody (HBF-719) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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