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Ly49F Antibody (HBF-719) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28097

Novus Biologicals, part of Bio-Techne
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NBP1-28097

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # HBF-719

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

CHO-K1 cells transfected with B6 allele of Ly49F gene

Specificity

B6 allele of the Ly49F receptor

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Ly49F Antibody (HBF-719) [FITC]

Flow Cytometry: Ly49F Antibody (HBF-719) [FITC] [NBP1-28097] - Analysis of C57BL/6 splenocytes by multiple staining.

Applications for Ly49F Antibody (HBF-719) [FITC]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Ly49F

The monoclonal antibody HBF-719 reacts with the B6 allele of Ly49F, a member of the lectin-like homodimeric Ly49 family of cell surface receptors.1 Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated. (2) These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells. The HBF-719 antibody stains approx. 11% of NK1.1+CD3- splenocytes, and 3.5% (2.5 month old) to 10.0% (11 month old) of CD8+ spleen cells from C57BL/6 mice. (1)

Alternate Names

Killer cell lectin like receptor 6, Klra6, Ly49F antigen, Lymphocyte antigen 49F, T cell surface glycoprotein Ly 49F

Gene Symbol

Klra6

Additional Ly49F Products

Product Documents for Ly49F Antibody (HBF-719) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Ly49F Antibody (HBF-719) [FITC]

Protect conjugated forms from light.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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