Lyp/PTPN22 Antibody (4F6)
Novus Biologicals, part of Bio-Techne | Catalog # H00026191-M01
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Porcine
Cited:
Human, Mouse
Applications
Validated:
ELISA, Knockdown Validated, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 4F6
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PTPN22 (AAH17785, 1 a.a. ~ 179 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDQREILQKFLDEAQSKKITKEEFANEFLKLKRQSTKYKADKTYPTTVAEKPKNIKKNRYKDILPYDYSRVELSLITSDEDSSYINANFIKGVYGPKAYIATQGPLSTTLLDFWRMIWEYSVLIIVMACMEYEMGKEAEKRKSDYIIRTLKVKFNSVSVILAHQTSLQNLFSQITPAHF
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 23619366). This antibody was tested in transfected lysates of mouse origin in PMID: 23619366. However, we have had mixed results with detection of the mouse protein in western blot.
Specificity
PTPN22 (4F6)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Lyp/PTPN22 Antibody (4F6)
![Western Blot: Lyp/PTPN22 Antibody (4F6) [H00026191-M01]](https://resources.bio-techne.com/images/products/Lyp-PTPN22-Antibody-4F6-Western-Blot-H00026191-M01-img0006.jpg "Western Blot: Lyp/PTPN22 Antibody (4F6) [H00026191-M01]")
Western Blot: Lyp/PTPN22 Antibody (4F6) [H00026191-M01]
Western Blot: Lyp/PTPN22 Antibody (4F6) [H00026191-M01] - Analysis of PTPN22 expression in transfected 293T cell line by PTPN22 monoclonal antibody (M01), clone 4F6.Lane 1: PTPN22 transfected lysate(85.1 KDa).Lane 2: Non-transfected lysate.![Western Blot: Lyp/PTPN22 Antibody (4F6) [H00026191-M01]](https://resources.bio-techne.com/images/products/Lyp-PTPN22-Antibody-4F6-Western-Blot-H00026191-M01-img0004.jpg "Western Blot: Lyp/PTPN22 Antibody (4F6) [H00026191-M01]")
Western Blot: Lyp/PTPN22 Antibody (4F6) [H00026191-M01]
Western Blot: Lyp/PTPN22 Antibody (4F6) [H00026191-M01] - Analysis of PTPN22 over-expressed 293 cell line, cotransfected with PTPN22 Validated Chimera RNAi ( Cat # H00026191-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PTPN22 monoclonal antibody (M01), clone 4F6 (Cat # H00026191-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.![ELISA: Lyp/PTPN22 Antibody (4F6) [H00026191-M01]](https://resources.bio-techne.com/images/products/Lyp-PTPN22-Antibody-4F6-ELISA-H00026191-M01-img0007.jpg "ELISA: Lyp/PTPN22 Antibody (4F6) [H00026191-M01]")
ELISA: Lyp/PTPN22 Antibody (4F6) [H00026191-M01]
ELISA: Lyp/PTPN22 Antibody (4F6) [H00026191-M01] - Detection limit for recombinant GST tagged PTPN22 is 1 ng/ml as a capture antibody.Applications for Lyp/PTPN22 Antibody (4F6)
Application
Recommended Usage
ELISA
1:100-1:2000
Western Blot
1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validaton and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Lyp
Long Name
Lymphoid Phosphatase
Alternate Names
LyPTP, PEP, PTPN22, PTPN8 (former)
Entrez Gene IDs
26191 (Human)
Gene Symbol
PTPN22
OMIM
152700 (Human)
UniProt
Additional Lyp Products
Product Documents for Lyp/PTPN22 Antibody (4F6)
Product Specific Notices for Lyp/PTPN22 Antibody (4F6)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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