Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1E12

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Lysine (K)-specific Demethylase 3A/KDM3A Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human JMJD1A expressed in E. coli. [UniProt# Q9Y4C1]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

147 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (1E12)Azide and BSA Free [NBP2-80839]

Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (1E12)Azide and BSA Free [NBP2-80839]

Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (1E12) - Azide and BSA Free [NBP2-80839] - Western blot analysis using JMJD1A mouse mAb against Hela (1) and HepG2 (2) cell lysates. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (1E12) - Azide and BSA Free [NBP2-80839]

Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (1E12) - Azide and BSA Free [NBP2-80839]

Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (1E12) - Azide and BSA Free [NBP2-80839] - JMJD1A antibody was tested at 1:10 in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (1E12) - Azide and BSA Free [NBP2-80839]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (1E12) - Azide and BSA Free [NBP2-80839]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (1E12) - Azide and BSA Free [NBP2-80839] - Immunohistochemical analysis of paraffin-embedded colonic cancer tissues (left) and lung cancer tissues (right) using JMJD1A mouse mAb with DAB staining. Image from the standard format of this antibody.
View 1 more images

Applications

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Simple Western

1:500

Western Blot

1:500-1:2000
Application Notes
This JMJD1A (1E12) antibody is useful in Western Blot, ELISA and Immunohistochemistry on paraffin-embedded sections.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 3A/KDM3A

JMJD1A is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. JMJD1A is involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. It's also Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.

Alternate Names

JHDM2A, JMJD1A, KDM3A, KIAA0742, Lysine (K)specific Demethylase 3A, TSGA

Gene Symbol

KDM3A

Additional Lysine (K)-specific Demethylase 3A/KDM3A Products

Product Documents

Product Datasheets

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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