Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Knockout Validated, Western Blot

Cited:

Chemotaxis, Flow Cytometry, IF/IHC, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Lysine (K)-specific Demethylase 3A/KDM3A Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1271 and 1321 of human jumonji domain containing 1A (JmjC domain-containing histone demethylation protein 2A) using the numbering given in entry NP_060903.2 (GeneID 55818).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24554764).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody

Knockout Validated: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282]

Knockout Validated: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282]

Knockout Validated: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282] - Total cell extracts of wild-type (RPE1WT) and KDM3A gene-edited RPE1 mutants (KDM3ACr22, named herein KDM3Anull) immunoblotted with KDM3A, revealing KDM3A-null clones (1, 2, and 8). Image collected and cropped by CiteAb from the following publication (https://rupress.org/jcb/article/216/4/999/52171/KDM3A-coordinates-actin-dynamics-with) licensed under a CC-BY license.
Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282]

Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282]

Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282] - Detection of Human JMJD1A/JHDM2A on HeLa whole cell lysate using NB100-77282. Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A was also immunoprecipitated by rabbit anti-Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A antibody NB100-77281.
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282]

Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282]

Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282] - Human cancer cells stained with NB100-77282 and Alexa Fluor 488 conjugated goat-anti-rabbit secondary antibody. DNA stained with Hoechst 33342. Staining shows KDM3A protein in mixed nuclear and cytoplasmic location. ICC/IF image submitted by a verified customer review.

Applications for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Use in chromatin immunoprecipitation reported in scientific literature (PMID: 19624751). Use in Immunohistochemistry reported in scientific literature (PMID: 30405805). This Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody is validated for ICC/IF from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NB100-77282 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Lysine (K)-specific Demethylase 3A/KDM3A

This gene encodes a zinc finger protein that contains a jumonji domain.

Alternate Names

JHDM2A, JMJD1A, KDM3A, KIAA0742, Lysine (K)specific Demethylase 3A, TSGA

Entrez Gene IDs

55818 (Human)

Gene Symbol

KDM3A

UniProt

Q9Y4C1

Additional Lysine (K)-specific Demethylase 3A/KDM3A Products

Product Documents for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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