Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52499

Novus Biologicals, part of Bio-Techne
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NBP2-52499
NBP2-52499-0.025mg
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6E10G4

Format

BSA Free

Concentration

1 mg/ml

View all Lysine (K)-specific Demethylase 4A/KDM4A Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A (AA: 932-1057) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

120.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4)BSA Free [NBP2-52499]

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4)BSA Free [NBP2-52499]

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) [NBP2-52499] - Western blot analysis using KDM4A mAb against human KDM4A (AA: 932-1057) recombinant protein. (Expected MW is 40.6 kDa)
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) - BSA Free [NBP2-52499]

Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) - BSA Free [NBP2-52499]

Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) [NBP2-52499] - Analysis of HeLa cells using KDM4A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4)BSA Free [NBP2-52499]

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4)BSA Free [NBP2-52499]

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) [NBP2-52499] - Analysis using KDM4A mAb against HEK293 (1) and KDM4A (AA: 932-1057)-hIgGFc transfected HEK293 (2) cell lysate.
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Applications

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 4A/KDM4A

JMJD2A is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein with a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.

Alternate Names

JHDM3A, JMJD2, JMJD2A, KDM4A, Lysine (K)specific Demethylase 4A

Gene Symbol

KDM4A

Additional Lysine (K)-specific Demethylase 4A/KDM4A Products

Product Documents

Product Datasheets

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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