Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52499
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 6E10G4
Format
BSA Free
Concentration
1 mg/ml
Product Summary
Immunogen
Purified recombinant fragment of human Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A (AA: 932-1057) expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
120.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images
Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4)BSA Free [NBP2-52499]
Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) [NBP2-52499] - Western blot analysis using KDM4A mAb against human KDM4A (AA: 932-1057) recombinant protein. (Expected MW is 40.6 kDa)Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) - BSA Free [NBP2-52499]
Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) [NBP2-52499] - Analysis of HeLa cells using KDM4A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4)BSA Free [NBP2-52499]
Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) [NBP2-52499] - Analysis using KDM4A mAb against HEK293 (1) and KDM4A (AA: 932-1057)-hIgGFc transfected HEK293 (2) cell lysate.Applications
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200-1:400
Immunocytochemistry/ Immunofluorescence
1:200-1:1000
Western Blot
1:500-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Lysine (K)-specific Demethylase 4A/KDM4A
Alternate Names
JHDM3A, JMJD2, JMJD2A, KDM4A, Lysine (K)specific Demethylase 4A
Gene Symbol
KDM4A
Additional Lysine (K)-specific Demethylase 4A/KDM4A Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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