Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (JE36-54)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32492
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE36-54
Concentration
1 mg/ml
Product Summary for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (JE36-54)
Immunogen
Recombinant protein within Human Lysine (K)-specific Demethylase 4AKDM4A/JMJD2A aa 551-650 / 1,064. (Uniprot: O75164)
Localization
Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
121 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (JE36-54)
Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (JE36-54) [NBP3-32492]
Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (JE36-54) [NBP3-32492] - Western blot analysis of Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A on different lysates with Rabbit anti-Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A antibody (NBP3-32492) at 1/1,000 dilution.Lane 1: MG-63 cell lysate (20 ug/Lane)
Lane 2: Mouse cerebellum tissue lysate (30 ug/Lane)
Lane 3: Mouse brain tissue lysate (30 ug/Lane)
Lane 4: Rat brain tissue lysate (30 ug/Lane)
Predicted band size: 121 kDa
Observed band size: 121 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32492) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
Applications for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (JE36-54)
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Lysine (K)-specific Demethylase 4A/KDM4A
Alternate Names
JHDM3A, JMJD2, JMJD2A, KDM4A, Lysine (K)specific Demethylase 4A
Gene Symbol
KDM4A
Additional Lysine (K)-specific Demethylase 4A/KDM4A Products
Product Documents for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (JE36-54)
Product Specific Notices for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (JE36-54)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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