Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP3-18688]

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP3-18688] - Figure 2. Western blot analysis using the antibody directed against Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Nuclear extracts from HeLa cells (40 ug) were analysed by Western blot using the antibody against Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size 121 kDa); the marker (in kDa) is shown on the left.

ELISA: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP3-18688]

ELISA: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP3-18688] - Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A , crude serum and flow through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,000.