Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (7H3D7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52500

Novus Biologicals, part of Bio-Techne
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NBP2-52500
NBP2-52500-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7H3D7

Format

BSA Free

Concentration

1 mg/ml

View all Lysine (K)-specific Demethylase 5B/KDM5B Antibodies »

Product Summary

Immunogen

Purified recombinant fragment of human Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B (AA: 231-319) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

175.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (7H3D7)BSA Free [NBP2-52500]

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (7H3D7)BSA Free [NBP2-52500]

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (7H3D7) [NBP2-52500] - Analysis using KDM5B mAb against human KDM5B (AA: 231-319) recombinant protein. (Expected MW is 36.2 kDa).
Flow Cytometry: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (7H3D7) - BSA Free [NBP2-52500]

Flow Cytometry: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (7H3D7) - BSA Free [NBP2-52500]

Flow Cytometry: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (7H3D7) [NBP2-52500] - Analysis of MCF-7 cells using KDM5B mouse mAb (green) and negative control (red).
Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (7H3D7)BSA Free [NBP2-52500]

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (7H3D7)BSA Free [NBP2-52500]

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (7H3D7) [NBP2-52500] - Against HEK293 (1) and KDM5B (AA: 231-319)-hIgGFc transfected HEK293 (2) cell lysate.
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Applications

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 5B/KDM5B

JARID1B (Jumonji AT-rich interactive domain 1B) is a member of the JARID 1 family of proteins that catalyze the demethylation of histones on lysine. The Jarid 1 family members include JARID1A, JARID1B, JARID1C and JARID1D. The members of this family possess a Jumonji C (JmjC) and Jumonji N (JmjN) domain as well as one ARID (AT-rich interactive domain) and three PHD-type zinc fingers. As histone demethylases, JARID 1 proteins function as transcriptional repressors. JARID1B expression is largely restricted to testis and may play a critical role in tumorigenesis as it has been found to be upregulated in prostate and breast cancer. Alternative names for JARID1B include retinoblastoma-binding protein 2 homolog 1, RBP2-H1, cancer/testis antigen 31, CT31, PLU-1, PUT1, and KDM5B.

Alternate Names

CT31, Jarid1B, KDM5B, Lysine (K)specific Demethylase 5B, PLU-1, PUT1, RBBP2H1

Gene Symbol

KDM5B

Additional Lysine (K)-specific Demethylase 5B/KDM5B Products

Product Documents

Product Datasheets

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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