Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP1-06640]

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP1-06640] - Total protein from human HepG2 and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-JMJD3 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free [NBP1-06640] -

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free [NBP1-06640] - KDM6A protein levels are decreased following EMT & upregulated during MET(A) HMLE cells were transduced with either an empty vector (HMLE-Vector), or genes encoding the EMT-inducing transcription factors Snail (HMLE-Snail) or Twist (HMLE-Twist) [4]. Whole-cell lysates were analyzed by immunoblotting for KDM6A. beta-actin was used as a loading control. (B-F) MCF10A cells were treated with sterile 4 mM HCl containing 0.1% bovine serum albumin (Vehicle) or TGFB for 5 days (TGFB+). In parallel, MCF10A cells were exposed to TGFB for 5 days in order to induce EMT, & subsequently subjected to TGFB-withdrawal, for a further 5 days, to elicit MET (TGFB+/-). (B-C) MCF10A cells, treated as described, were fixed & immunostained for E-cadherin (B) & N-cadherin (C). Nuclei were counterstained with DAPI (blue). Scale bars, 50 μm. (D) MCF10A cells, treated as described, were analyzed by immunoblotting for KDM6B & vimentin (Vim). COX IV was used as a loading control. (E) MCF10A cells, treated as described, were fixed & immunostained for KDM6A. Nuclei were counterstained with DAPI (blue). Scale bars, 50 μm. (F) Cells were analyzed by immunoblotting for KDM6A, EZH2 & RING1A. beta-actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.19214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.