Lysozyme Antibody (A5A8-R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32552
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # A5A8-R
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within Human Lysozyme aa 1-141 / 148. (Uniprot: P61626)
Localization
Secreted.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Lysozyme Antibody (A5A8-R)
Western Blot: Lysozyme Antibody (A5A8-R) [NBP3-32552]
Western Blot: Lysozyme Antibody (A5A8-R) [NBP3-32552] - Western blot analysis of Lysozyme on different lysates with Mouse anti-Lysozyme antibody (NBP3-32552) at 1/1,000 dilution.Lane 1: HL-60 cell lysate
Lane 2: U-937 cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 17 kDa
Observed band size: 14 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32552) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry: Lysozyme Antibody (A5A8-R) [NBP3-32552]
Immunohistochemistry: Lysozyme Antibody (A5A8-R) [NBP3-32552] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-Lysozyme antibody (NBP3-32552) at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32552) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Applications for Lysozyme Antibody (A5A8-R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:1000
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Lysozyme
Alternate Names
EC 3.2.1.17, lysozyme, lysozyme (renal amyloidosis)1,4-beta-N-acetylmuramidase C, lysozyme C, LZM, renal amyloidosis
Gene Symbol
LYZ
Additional Lysozyme Products
Product Documents for Lysozyme Antibody (A5A8-R)
Product Specific Notices for Lysozyme Antibody (A5A8-R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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