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Lysozyme Antibody (BGN/0696/5B1)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-63062

Novus Biologicals, part of Bio-Techne
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NB100-63062
NB100-63062-0.025mg

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # BGN/0696/5B1

Concentration

1 mg/ml

Product Specifications

Immunogen

Native, from urine

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34099671).

Localization

Secreted

Specificity

NB100-63062 recognizes lysozyme, an enzyme that is present in mucosal secretions such as saliva and tears and is involved with non-specific organism defence. It functions by hydrolyzing 1,2-beta linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues such as those found in the walls of various Gram-positive bacteria.

Marker

Histiocytic Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Lysozyme Antibody (BGN/0696/5B1)

CyTOF-ready: Lysozyme Antibody (BGN/0696/5B1) [NB100-63062] - Human bone marrow after RBC lysis was stained with surface antibodies, fixed, permeabilized with methanol, stained with anti-lysozyme NB100-63062, and gated for the above populations per Nat Med. 2020. 26:408-417. CyTOF image submitted by a verified customer review.

Applications for Lysozyme Antibody (BGN/0696/5B1)

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 29930984). This Lysozyme antibody (BGN/0696/5B1) is validated for CyTOF from a verified customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB100-63062 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysozyme

Lysozyme catalyzes the hydrolysis of certain mucopolysaccharides of bacterial cell walls. Specifically, it catalyzes the hydrolysis of the bacterial cell wall beta glycosidic linkages between N acetylmuramic acid and N acetylglucosamine. It is found in spleen, lung, kidney, white blood cells, plasma, saliva, milk, and tears.

Alternate Names

EC 3.2.1.17, lysozyme, lysozyme (renal amyloidosis)1,4-beta-N-acetylmuramidase C, lysozyme C, LZM, renal amyloidosis

Entrez Gene IDs

4069 (Human)

Gene Symbol

LYZ

UniProt

Additional Lysozyme Products

Product Documents for Lysozyme Antibody (BGN/0696/5B1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lysozyme Antibody (BGN/0696/5B1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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