LYVE-1 Antibody (RM0033-4D17) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (RM0033-4D17) - Azide and BSA Free [NB110-61026] -

Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (RM0033-4D17) - Azide and BSA Free [NB110-61026] - Mouse LLECs develop independent of the transcription factor PU.1. a–d As detected by IHC, E15.5 wild-type mice have cells that co-express LYVE1 (a, red) PROX1 (b, green) & MRC1 (c, white) in the developing meninges (white bracket). DAPI labels nuclei blue in the merged image (d). White arrows indicate cells with these three markers. Scale = 50 µm. n = 2 brains. e–h) The meninges (white bracket) of E15.5 PU.1 knockout siblings contain many cells (white arrows) that co-express LYVE1 (e, red), PROX1 (f, green), & MRC1 (g, white). Scale = 50 µm. n = 3 brains Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (RM0033-4D17) - Azide and BSA Free [NB110-61026] -

Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (RM0033-4D17) - Azide and BSA Free [NB110-61026] - Mouse LLECs take up A beta 1-40. a Schematic showing the site of dye & A beta1-40 perfusion into the CSF via the cisterna magna (arrow) of a 2-month old mouse. The dotted line indicates the plane of section. A anterior, P posterior, D dorsal, V ventral. b Coronal brain section indicating the areas imaged. SF4 refers to area captured in Figure S4. c The percentage of each labelled cell type that internalized perfused A beta. Cells co-expressing VEGFR3 & LYVE1 take up A beta at a higher rate than MRC1, LYVE1 double-positive cells as well as MRC1-positive, LYVE1-negative cells (p ≤ 0.05, bootstrap). VEGFR3, LYVE1 counts, n = 2 brains (3 sections/brain). MRC1, LYVE1 counts, n = 3 brains (3 sections/brain). d–d′′′ Cells of the adult mouse meninges that co-express VEGFR3 (d, green) & LYVE1 (d′, white) internalize A beta1-40 (d′′, cyan). Scale = 20 µm. e-e′′′) Cells of the adult mouse meninges that co-express VEGFR3 (e, green) & MRC1 (e′, white) internalize A beta1-40 (e′′, cyan). Scale = 40 µm. f–f′′′) Cells of the adult mouse meninges that co-express MRC1 (f, magenta) & LYVE1 (f′, white) internalize A beta1-40 (f′′, cyan). The walls of a blood vessel (white arrowhead, f′′) also accumulate A beta1-40. Scale = 60 µm Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (RM0033-4D17) - Azide and BSA Free [NB110-61026] -

Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (RM0033-4D17) - Azide and BSA Free [NB110-61026] - Cells with BLEC molecular markers are present within the mouse leptomeninges. a Coronal brain section of adult zebrafish brain indicating the imaging area in the dorsal optic tectum (TeO). b A 14 month old Tg(kdr-l:mCherry); Tg(flt4:mCitrine) double transgenic zebrafish has cells in the meninges (white bracket) that express flt4/vegfr3 ( alpha-GFP, green) near kdr-l positive ( alpha-RFP, red) blood vessels. DAPI (blue) labels the nuclei. Scale = 50 µm. c Coronal mouse brain section showing the imaging areas of the meninges. d As revealed by IHC, 17-week-old mouse brains express VEGFR3 (green) in the meninges (white bracket). Tie2-GFP;NG2-DsRed double reporter mice were used to distinguish arteries & veins. NG2 (red) labels pericytes & smooth muscle cells, Tie2 (magenta) labels vascular endothelial cells, & Hoechst (blue) stains nuclei. The image is rotated with the parenchyma at the bottom for ease of comparison with panel b. Scale = 50 µm. e-e′′′ As revealed by IHC, cells of the meninges co-express MRC1 (e, yellow), LYVE1 (e′, white), & VEGFR3 (e′′, green). Red arrows highlight cells expressing these three markers. The images are rotated with the parenchyma at the bottom. scale = 30 µm. f, g Quantification of the relative numbers of single & double-labelled cells in 2-month old mouse meninges. VEGFR3 & LYVE1 cell counts were from n = 2 brains, 3 coronal sections (10 area images)/brain. MRC1 & LYVE1 cell counts were from n = 3 brains, 3 coronal sections (4 area images)/brain. The mean values for each set are depicted Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (RM0033-4D17) - Azide and BSA Free [NB110-61026] -

Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (RM0033-4D17) - Azide and BSA Free [NB110-61026] - Cells of human meninges co-express LLEC markers. a–c DAB-IHC with single antibodies detects VEGFR3 (a), LYVE1 (b), & MRC1 (c) in the meninges of human post mortem brain showing no signs of neuropathology. These images are taken from a 38 year old male (sample P17/07, Table 1), & confirmed in n = 2 additional samples. P parenchyma. Scale = 150 µm (a); 40 µm (b); & 20 µm (c). d–f DAB-IHC with single antibodies detects VEGFR3 (b), LYVE1 (c), & MRC1 (d) in elderly human meninges (age: 89–92) with evidence of neuropathology & confirmed in n = 3 brains (Table 1). P, parenchyma. Scale = 20 µm. g–p IHC with fluorescent antibodies detects human meningeal cells that co-express MRC1 (h, m, yellow), LYVE1 (i, n, white), & VEGFR3 (j, o, green). Nuclei/RNA are labelled with DAPI (g, l, blue) & images are merged in (k, p). Scale = 10 µm Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (RM0033-4D17) - Azide and BSA Free [NB110-61026] -

Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (RM0033-4D17) - Azide and BSA Free [NB110-61026] - Cells expressing LLEC markers are present within embryonic mouse leptomeninges. a–d As revealed by IHC, E12.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), & MRC1 (white). DAPI marks the nuclei in blue (d). Scale = 30 µm. n = 3 brains. e–h E13.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), & MRC1 (white). DAPI marks the nuclei in blue (h). Scale = 30 µm. n = 3 brains. i–l E18 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), & MRC1 (white). PROX1 is cytoplasmic (j, red arrows) in the meninges but nuclear in neurons (j, blue arrows). DAPI marks the nuclei in blue (l). Scale = 30 µm. n = 3 brains Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.