Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunoprecipitation (Negative), Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all MACF1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1975 to 2025 of human Microtubule-Actin Crosslinking Factor 1 using the numbering given in entry NP_036222.3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MACF1 Antibody

Western Blot: MACF1 Antibody [NBP2-36528]

Western Blot: MACF1 Antibody [NBP2-36528]

Western Blot: MACF1 Antibody [NBP2-36528] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MACF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for MACF1 Antibody

Application
Recommended Usage

Western Blot

1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MACF1

MACF1, also known as microtubule-actin cross-linking factor 1, isoforms 1/2/3/5, consists of five isoforms of sizes 838.3 kDa, 620.4 kDa, 614.1 kDa, 606.2 kDa, and 670.1 kDa and is involved in binding and cross-linking actin to microtubules and may play a role in transporting organelles throughout the cell. Diseases and disorders such as hepatitis, neuroblastoma, bullous pemphigoid, and schizophrenia are being researched with this protein. The protein interacts with SKIL, GOLGA4, ATF7IP, DISC1, and DTNBP1.

Alternate Names

microtubule-actin crosslinking factor 1

Gene Symbol

MACF1

UniProt

NP_036222.3 (Human)

Additional MACF1 Products

Product Documents for MACF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MACF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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