MAD2L2 Antibody (4D8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26158
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4D8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human MAD2L2 [UniProt Q9UI95]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MAD2L2 Antibody (4D8)
Immunohistochemistry: MAD2L2 Antibody (4D8) [NBP3-26158] -
Immunohistochemistry: MAD2L2 Antibody (4D8) [NBP3-26158] - Image of MAD2L2 Antibody (4D8) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: MAD2L2 Antibody (4D8) [NBP3-26158] -
Western Blot: MAD2L2 Antibody (4D8) [NBP3-26158] - Positive Western Blot detected in: 293 whole cell lysate.All lanes: MAD2L2 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Immunocytochemistry/Immunofluorescence: MAD2L2 Antibody (4D8) [NBP3-26158] -
Immunocytochemistry/Immunofluorescence: MAD2L2 Antibody (4D8) [NBP3-26158] - Staining of Hela Cells with MAD2L2 Antibody (4D8) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).Applications for MAD2L2 Antibody (4D8)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: MAD2L2
Alternate Names
hREV7, MAD2 mitotic arrest deficient-like 2 (yeast), MAD2BREV7 homolog, MAD2-like protein 2, Mitotic arrest deficient 2-like protein 2, mitotic arrest deficient homolog-like 2, mitotic spindle assembly checkpoint protein MAD2B, REV7yeast, homolog)-like 2
Gene Symbol
MAD2L2
Additional MAD2L2 Products
Product Documents for MAD2L2 Antibody (4D8)
Product Specific Notices for MAD2L2 Antibody (4D8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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