MAD2L2 Antibody (7B6I2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16451

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16451-100ul
NBP3-16451-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7B6I2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MAD2L2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human MAD2B/MAD2L2 (Q9UI95). FEITQPPLLSISSDSLLSHVEQLLRAFILKISVCDAVLDHNPPGCTFTVLVHTREAATRNMEKIQVIKDFPWILADEQDVHMHDPRLIPLKTMTSDILKMQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAD2L2 Antibody (7B6I2)

Western Blot: MAD2L2 Antibody (7B6I2) [NBP3-16451]

Western Blot: MAD2L2 Antibody (7B6I2) [NBP3-16451]

Western Blot: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Western blot analysis of extracts of various cell lines, using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: MAD2L2 Antibody (7B6I2) [NBP3-16451]

Immunocytochemistry/ Immunofluorescence: MAD2L2 Antibody (7B6I2) [NBP3-16451]

Immunocytochemistry/Immunofluorescence: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Immunofluorescence analysis of NIH-3T3 cells using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: MAD2L2 Antibody (7B6I2) [NBP3-16451]

Immunohistochemistry-Paraffin: MAD2L2 Antibody (7B6I2) [NBP3-16451]

Immunohistochemistry-Paraffin: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Immunohistochemistry of paraffin-embedded mouse brain using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for MAD2L2 Antibody (7B6I2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MAD2L2

MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1.

Alternate Names

hREV7, MAD2 mitotic arrest deficient-like 2 (yeast), MAD2BREV7 homolog, MAD2-like protein 2, Mitotic arrest deficient 2-like protein 2, mitotic arrest deficient homolog-like 2, mitotic spindle assembly checkpoint protein MAD2B, REV7yeast, homolog)-like 2

Gene Symbol

MAD2L2

Additional MAD2L2 Products

Product Documents for MAD2L2 Antibody (7B6I2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MAD2L2 Antibody (7B6I2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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