Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

PE/Cy7 (Excitation = 488 nm, Emission = 778 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 683715

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MAdCAM-1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MAdCAM-1
Leu21-Gln333
Accession # AAY82472

Specificity

Detects human MAdCAM-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse MAdCAM-1, recombinant human (rh) ALCAM, rhBCAM, rhCEACAM-1, rhEpCAM, rhICAM-1, -2, -3, -4, -5, rhCD31/PECAM-1, or rhVCAM-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for MAdCAM-1 Antibody (683715) [PE/Cy7]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: MAdCAM-1

Mucosal vascular addressin-1 (MAdCAM-1) is a type I transmembrane glycoprotein expressed at high levels on high endothelial venules (HEV) of Peyer's patches and mesenteric lymph nodes, and on flat walled venules within the gut lamina propria. It is also expressed on sinus-lining cells in the spleen. (1-3) The countereceptor or "homing receptor" for MAdCAM-1 is alpha4beta7 integrin (also known as LPAM-1). MAdCAM-1 is also a facultative ligand for CD62L/L-selectin. (4, 5) The monoclonal antibody MECA-367 binds to the first domain of MAdCAM-1 and blocks MAdCAM-1-dependent binding in vitro and lymphocyte homing to Peyer's patch HEV in vivo. (1, 2, 4)

Long Name

Mucosal Addressin Cell Adhesion Molecule 1

Alternate Names

MAdCAM1

Gene Symbol

MADCAM1

Additional MAdCAM-1 Products

Product Documents for MAdCAM-1 Antibody (683715) [PE/Cy7]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MAdCAM-1 Antibody (683715) [PE/Cy7]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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