MafA Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB400-137
![Western Blot: MafA Antibody [NB400-137] Western Blot: MafA Antibody [NB400-137]](https://resources.bio-techne.com/images/products/MafA-Antibody-Western-Blot-NB400-137-img0008.jpg)
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Gel Super Shift Assays, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Block/Neutralize, Chemotaxis, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 300 and the C-terminus (residue 359) of mouse v-maf musculoaponeurotic fibrosarcoma oncogene homolog A using the numbering given in entry NP_919331.1 (GeneID 378435).
Reactivity Notes
Human reactivity reported in the scientific literature (PMID: 22665223).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MafA Antibody - BSA Free
Western Blot: MafA Antibody [NB400-137]
Western Blot: MafA Antibody [NB400-137] - Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or cMaf expression constructs. Affinity purified anti-MafA antibody (NB400-137), anti-MafB antibody (NB600-266) or anti-c-Maf antibody (NB600-267). Each antibody was used at 0.5 ug/ml.Immunohistochemistry-Paraffin: MafA Antibody [NB400-137]
Immunohistochemistry-Paraffin: MafA Antibody [NB400-137] - Immunohistochemical staining of MafA (blue) and insulin (green) in mouse pancreas. Image from verified customer review.Western Blot: MafA Antibody [NB400-137]
Western Blot: MafA Antibody [NB400-137] - Binding of MafA to the enhancer region of the endogenous insulin gene. Samples: Immunoprecipitated cross-linked DNA from betaTC-3 cells was analyzed by PCR. As controls, reactions were run with no DNA, with input chromatin, with DNA obtained after precipitation with rabbit IgG or without antibody (lanes 1 through 5, respectively). Antibody: Affinity purified rabbit anti-MafA antibody (NB 400-137).Applications for MafA Antibody - BSA Free
Application
Recommended Usage
Gel Super Shift Assays
1 to 5 ug/20 ul reaction
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:1000 to 1:3000
Western Blot
1:1000-1:10000
Application Notes
A band ~36-40 kDa is seen in WB analysis. Use in IHC-P was reported in the scientific literature (PMID: 22665223). In some cases, the antibody may be diluted further than indicated. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Use in ICC/IF and in IHC-Frozen reported in scientific literature (PMID 23967072). Chip reported in scientific literature (PMID: 26314560).
Reviewed Applications
Read 1 review rated 5 using NB400-137 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MafA
Alternate Names
hMafA, Pancreatic beta-cell-specific transcriptional activator, RIPE3B1, transcription factor MafA, Transcription factor RIPE3b1, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A, v-maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian)
Entrez Gene IDs
378435 (Mouse)
Gene Symbol
MAFA
UniProt
Additional MafA Products
Product Documents for MafA Antibody - BSA Free
Product Specific Notices for MafA Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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