Skip to main content

MafB Antibody (OTI1E9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45718

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP2-45718
Conjugate
Catalog #

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1E9

Concentration

1 mg/ml

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 32-320 of human MAFB (NP_005452) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

35.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MafB Antibody (OTI1E9)

Western Blot: MafB Antibody (OTI1E9) [NBP2-45718]

Western Blot: MafB Antibody (OTI1E9) [NBP2-45718]

Western Blot: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAFB.
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]

Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]

Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Carcinoma of Human lung tissue.
Flow Cytometry: MafB Antibody (OTI1E9) [NBP2-45718]

Flow Cytometry: MafB Antibody (OTI1E9) [NBP2-45718]

Flow Cytometry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by MAFB antibody.

Applications for MafB Antibody (OTI1E9)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:2000
Application Notes
Heat-induced epitope retrieval by 10mM citric buffer, pH 6.0, 100C for 10 min is recommended

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MafB

MAFB is encoded by this gene is a basic leucine zipper (bZIP) transcription factor that plays an important role in the regulation of lineage-specific hematopoiesis. The encoded nuclear protein represses ETS1-mediated transcription of erythroid-specific genes in myeloid cells. This gene contains no introns.

Long Name

v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog B

Alternate Names

KRML

Gene Symbol

MAFB

Additional MafB Products

Product Documents for MafB Antibody (OTI1E9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MafB Antibody (OTI1E9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...
Loading...