MafB Antibody (OTI1E9) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72569
![Western Blot: MafB Antibody (OTI1E9)Azide and BSA Free [NBP2-72569]](https://resources.bio-techne.com/images/products/MafB-Antibody-OTI1E9-Azide-and-BSA-Free-Western-Blot-NBP2-72569-img0001.jpg "Western Blot: MafB Antibody (OTI1E9)Azide and BSA Free [NBP2-72569]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI1E9
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 32-320 of human MAFB (NP_005452) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
35.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MafB Antibody (OTI1E9) - Azide and BSA Free
Western Blot: MafB Antibody (OTI1E9)Azide and BSA Free [NBP2-72569]
Western Blot: MafB Antibody (OTI1E9) - Azide and BSA Free [NBP2-72569] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAFB.![Flow Cytometry: MafB Antibody (OTI1E9) - Azide and BSA Free [NBP2-72569]](https://resources.bio-techne.com/images/products/MafB-Antibody-OTI1E9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72569-img0002.jpg "Flow Cytometry: MafB Antibody (OTI1E9) - Azide and BSA Free [NBP2-72569]")
Flow Cytometry: MafB Antibody (OTI1E9) - Azide and BSA Free [NBP2-72569]
Flow Cytometry: MafB Antibody (OTI1E9) - Azide and BSA Free [NBP2-72569] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by MAFB antibody.Applications for MafB Antibody (OTI1E9) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Western Blot
1:2000
Application Notes
Heat-induced epitope retrieval by 10mM citric buffer, pH 6.0, 100C for 10 min is recommended
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MafB
Long Name
v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog B
Alternate Names
KRML
Gene Symbol
MAFB
Additional MafB Products
Product Documents for MafB Antibody (OTI1E9) - Azide and BSA Free
Product Specific Notices for MafB Antibody (OTI1E9) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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