MafK Antibody (2F7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22570

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F7

Format

BSA Free

Concentration

1 mg/ml

View all MafK Antibodies »

Product Summary for MafK Antibody (2F7) - BSA Free

Immunogen

Recombinant human MAFK (1-156aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for MafK Antibody (2F7) - BSA Free

Western Blot: MafK Antibody (2F7)BSA Free [NBP2-22570]

Western Blot: MafK Antibody (2F7)BSA Free [NBP2-22570]

Western Blot: MafK Antibody (2F7) [NBP2-22570] - Western Blot: MafK Antibody (2F7) [NBP2-22570] - The recombinant proteins (20ng) were resolved by SDSPAGE, transferred to PVDF membrane and probed with anti human MAFK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : Recombinant Human MAFKLane 2. : Recombinant Human MAFGLane 3. : Recombinant Human MAFF
Western Blot: MafK Antibody (2F7)BSA Free [NBP2-22570]

Western Blot: MafK Antibody (2F7)BSA Free [NBP2-22570]

Western Blot: MAFK Antibody (2F7) [NBP2-22570] - The cell lysates of Jurkat (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAFK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Applications for MafK Antibody (2F7) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MafK

The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2). NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG or MafK) and p45. Since Maf homodimers lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. The MafK of Maf proteins is primarily expressed during development in mesenchymal and hematopoietic cells and neurons.

Long Name

v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog K

Alternate Names

NFE2U

Gene Symbol

MAFK

UniProt

NP_002351 (Human)

Additional MafK Products

Product Documents for MafK Antibody (2F7) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MafK Antibody (2F7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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