MAGED2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89413

Novus Biologicals, part of Bio-Techne
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NBP1-89413
NBP1-89413-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MAGED2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LTDTQVLAAENKSLAADTKKQNADPQAVTMPATETKKVSHVADTKVNTKAQETEAAPSQAPADEPEPESAAAQSQENQDTR

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25663882).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAGED2 Antibody

Western Blot: MAGED2 Antibody [NBP1-89413]

Western Blot: MAGED2 Antibody [NBP1-89413]

Western Blot: MAGED2 Antibody [NBP1-89413] - Analysis in control (vector only transfected HEK293T lysate) and MAGED2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: MAGED2 Antibody [NBP1-89413]

Immunocytochemistry/ Immunofluorescence: MAGED2 Antibody [NBP1-89413]

Immunocytochemistry/Immunofluorescence: MAGED2 Antibody [NBP1-89413] - Staining of human cell line U-2 OS shows positivity in nucleus, nucleoli and cytoplasm.
Immunohistochemistry-Paraffin: MAGED2 Antibody [NBP1-89413]

Immunohistochemistry-Paraffin: MAGED2 Antibody [NBP1-89413]

Immunohistochemistry-Paraffin: MAGED2 Antibody [NBP1-89413] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.

Applications for MAGED2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAGED2

MAGED2 was identified as a gene involved in X-linked mental retardation. MAGED2 is a member of the MAGED gene family whose members are clustered on chromosome Xp11.

Alternate Names

11B6MAGE-D2 antigen, BCG-1, BCG1MAGEDHCA10, breast cancer associated gene 1, Breast cancer-associated gene 1 protein, hepatocellular carcinoma associated protein, hepatocellular carcinoma-associated protein HCA10, Hepatocellular carcinoma-associated protein JCL-1, JCL-1, MAGE-D2, melanoma antigen family D, 2, melanoma-associated antigen D2, MGC8386

Gene Symbol

MAGED2

Additional MAGED2 Products

Product Documents for MAGED2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MAGED2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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