MAK Antibody (3E5)

Novus Biologicals, part of Bio-Techne | Catalog # H00004117-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3E5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MAK Antibodies »

Product Specifications

Immunogen

MAK (AAH39825, 358 a.a. ~ 457 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPKQQSQEKPPQTLFPSIVKNMPTKPNGTLSHKSGRRRWGQTIFKSGDSWEELEDYDFGASHSKKPSMGVFKEKRKKDSPFRQQVKMAVISLSAHQFPTL

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

MAK (3E5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MAK Antibody (3E5)

Western Blot: MAK Antibody (3E5) [H00004117-M01]

Western Blot: MAK Antibody (3E5) [H00004117-M01]

Western Blot: MAK Antibody (3E5) [H00004117-M01] - MAK monoclonal antibody (M01), clone 3E5. Analysis of MAK expression in NIH/3T3.
Immunocytochemistry/ Immunofluorescence: MAK Antibody (3E5) [H00004117-M01]

Immunocytochemistry/ Immunofluorescence: MAK Antibody (3E5) [H00004117-M01]

Immunocytochemistry/Immunofluorescence: MAK Antibody (3E5) [H00004117-M01] - Analysis of monoclonal antibody to MAK on HepG2 cell. Antibody concentration 10 ug/ml.
Western Blot: MAK Antibody (3E5) [H00004117-M01]

Western Blot: MAK Antibody (3E5) [H00004117-M01]

Western Blot: MAK Antibody (3E5) [H00004117-M01] - MAK monoclonal antibody (M01), clone 3E5. Analysis of MAK expression in PC-12.
View 2 more images

Applications for MAK Antibody (3E5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MAK

The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. It is expressed almost exclusively in the testis, primarily in germ cells. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. There is, however, a study of the mouse homolog that has identified high levels of expression in developing sensory epithelia so its function may be more generalized.

Alternate Names

dJ417M14.2, EC 2.7.11, EC 2.7.11.22, male germ cell-associated kinaseserine/threonine protein kinase MAK, serine/threonine-protein kinase MAK

Entrez Gene IDs

4117 (Human)

Gene Symbol

MAK

OMIM

154235 (Human)

UniProt

AAH39825 (Human)

Additional MAK Products

Product Documents for MAK Antibody (3E5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MAK Antibody (3E5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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