Maltose Binding Protein Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97370
Key Product Details
Species Reactivity
Non-species specific
Applications
Validated:
ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This Maltose Binding Protein Antibody was purified from whole rabbit serum prepared by repeated immunizations with the Maltose Binding Protein epitope tag recombinant protein.
Specificity
This IgG purified antibody is directed against MBP and is useful in determining its presence in various assays. This polyclonal anti-MBP tag antibody detects over-expressed proteins containing the MBP epitope tag. To date this antibody has reacted with all MBP tagged proteins so far tested. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This IgG purified antibody is directed against Maltose Binding Protein and is useful in determining its presence in various assays
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Maltose Binding Protein Antibody - BSA Free
Western Blot: Maltose Binding Protein AntibodyBSA Free [NBP1-97370]
Western Blot: Maltose Binding Protein Antibody - BSA Free [NBP1-97370] - Western Blot of Maltose Binding protein antibody - BSA Free.Lane 1: 1.0 ug of recombinant protein containing the Maltose Binding Protein.Primary Antibody: Polyclonal rabbit-anti-Maltose Binding Protein at 0.5-1.0 ug/ml for 1 h at room temperature.Secondary Antibody: 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature. Imaging: LICOR's Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Predicted MW: ~42 kDa. A minor band at corresponding to multimers of this protein is also evident.Western Blot: Maltose Binding Protein AntibodyBSA Free [NBP1-97370]
Western Blot: Maltose Binding Protein Antibody - BSA Free [NBP1-97370] - Western Blot showing detection of Maltose Binding protein antibody - BSA Free.Lane 1: MW markers. Lane 2: Maltose Binding Protein [0.05 ug]. Protein was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. Blocking with 1% BSA-TTBS 30 min at 20C. Primary Antibody: Anti-MBP (RABBIT) antibody was used at 1:1000 overnight at 4C. Secondary Antibody: Anti-Rabbit IgG (GOAT) IRDye800(R) conjugated antibody was used at 1:20,000 in Blocking Buffer for Fluorescent Western Blotting for 30 min at 20C. Imaged on the LiCor Odyssey imaging system. Predicted MW: ~42 kDa, the other bands present are recombinant MBP breakdown.
N/A: Maltose Binding Protein Antibody - BSA Free [NBP1-97370]
Applications for Maltose Binding Protein Antibody - BSA Free
Application
Recommended Usage
ELISA
1:10000-1:50000
Western Blot
1:1000-1:5000
Application Notes
This product is optimally suited for monitoring the expression of MBP tagged fusion proteins. As such, anti-MBP/MBP can be used to identify fusion proteins containing the MBP epitope. The antibody recognizes the MBP epitope tag fused to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against MBP containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques. Maltose binding protein is a bacterial protein, which is often used in protein expression studies because it creates a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest. It also allows for its easy purification from bacterial extracts under mild conditions. This product is a companion to the pMAL protein expression system and can be used for the detection and purification of MBP-fusion proteins expressed in E. coli. By Western blot, a band is seen at ~ 42 kDa representing MBP.
Formulation, Preparation, and Storage
Purification
IgG purified
Reconstitution
For the 1mg size, we recommend reconstitution with 1.0 ml of deionized water. The 0.5mg size is provided in liquid format (0.5 ml)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Maltose Binding Protein
Alternate Names
Mal E, MalE, Maltodextrin binding protein, Maltose ABC transporter periplasmic protein, Maltose binding periplasmic protein, MBP, MMBP, Periplasmic maltose binding protein
Additional Maltose Binding Protein Products
Product Documents for Maltose Binding Protein Antibody - BSA Free
Product Specific Notices for Maltose Binding Protein Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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