MAN1A2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82802

Novus Biologicals, part of Bio-Techne
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NBP1-82802
NBP1-82802-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MAN1A2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KLRKSREEIRAEIQTEKNKVVQEMKIKENKPLPPVPIPNLVGIRGGDPEDNDIREK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAN1A2 Antibody

Immunohistochemistry-Paraffin: MAN1A2 Antibody [NBP1-82802]

Immunohistochemistry-Paraffin: MAN1A2 Antibody [NBP1-82802]

Immunohistochemistry-Paraffin: MAN1A2 Antibody [NBP1-82802] - Staining in human placenta and skeletal muscle tissues . Corresponding MAN1A2 RNA-seq data are presented for the same tissues.
Western Blot: MAN1A2 Antibody [NBP1-82802]

Western Blot: MAN1A2 Antibody [NBP1-82802]

Western Blot: MAN1A2 Antibody [NBP1-82802] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-330
Immunocytochemistry/ Immunofluorescence: MAN1A2 Antibody [NBP1-82802]

Immunocytochemistry/ Immunofluorescence: MAN1A2 Antibody [NBP1-82802]

Immunocytochemistry/Immunofluorescence: MAN1A2 Antibody [NBP1-82802] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.
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Applications for MAN1A2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 24591635). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP1-82802 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAN1A2

Alpha-mannosidases function at different stages of N-glycan maturation in mammalian cells. See MAN2A1 (MIM 154582) for general information.[supplied by OMIM]

Long Name

Mannosidase alpha, Class 1A, Member 2

Alternate Names

MAN1B

Entrez Gene IDs

10905 (Human)

Gene Symbol

MAN1A2

UniProt

O60476 (Human)

Additional MAN1A2 Products

Product Documents for MAN1A2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MAN1A2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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