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MANBA Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-04803

Novus Biologicals, part of Bio-Techne
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NBP3-04803-100ul
NBP3-04803-20ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for MANBA Antibody - Azide and BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 18-300 of human MANBA (NP_005899.3). AAELSYSLRGNWSICNGNGSLELPGAVPGCVHSALFQQGLIQDSYYRFNDLNYRWVSLDNWTYSKEFKIPFEISKWQKVNLILEGVDTVSKILFNEVTIGETDNMFNRYSFDITNVVRDVNSIELRFQSAVLYAAQQSKAHTRYQVPPDCPPLVQKGECHVNFVRKEQCSFSWDWGPSFPTQGIWKDVRIEAYNICHLNYFTFSPIYDKSAQEWNLEIESTFDVVSSKPVGGQVIVAIPKLQTQQTYSIELQPGKRIVELFVNISKNITVETWWPHGHGNQTG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MANBA Antibody - Azide and BSA Free

Western Blot: MANBA AntibodyAzide and BSA Free [NBP3-04803]

Western Blot: MANBA AntibodyAzide and BSA Free [NBP3-04803]

Western Blot: MANBA Antibody [NBP3-04803] - Analysis of extracts of various cell lines, using MANBA antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit
Immunohistochemistry-Paraffin: MANBA Antibody - Azide and BSA Free [NBP3-04803]

Immunohistochemistry-Paraffin: MANBA Antibody - Azide and BSA Free [NBP3-04803]

Immunohistochemistry-Paraffin: MANBA Antibody [NBP3-04803] - Paraffin-embedded human liver cancer using MANBA antibody at dilution of 1:100 (40x lens).
Immunohistochemistry-Paraffin: MANBA Antibody - Azide and BSA Free [NBP3-04803]

Immunohistochemistry-Paraffin: MANBA Antibody - Azide and BSA Free [NBP3-04803]

Immunohistochemistry-Paraffin: MANBA Antibody [NBP3-04803] - Paraffin-embedded human thyroid cancer using MANBA antibody at dilution of 1:100 (40x lens).

Applications for MANBA Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:200-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MANBA/Beta-Mannosidase

MANBA encodes a member of the glycosyl hydrolase 2 family. The encoded protein localizes to the lysosome where it is the final exoglycosidase in the pathway for N-linked glycoprotein oligosaccharide catabolism. Mutations in this gene are associated with beta-mannosidosis, a lysosomal storage disease that has a wide spectrum of neurological involvement.

Long Name

Beta-Mannosidase

Alternate Names

MANB1, Mannanase, Mannase

Gene Symbol

MANBA

Additional MANBA/Beta-Mannosidase Products

Product Documents for MANBA Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MANBA Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov