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Mannose Phosphate Isomerase Antibody (OTI1C7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02043

Novus Biologicals, part of Bio-Techne
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NBP2-02043
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1C7

Concentration

1 mg/ml

Product Summary for Mannose Phosphate Isomerase Antibody (OTI1C7)

Immunogen

Full length human recombinant protein of human MPI(NP_002426) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Mannose Phosphate Isomerase Antibody (OTI1C7)

Western Blot: Mannose Phosphate Isomerase Antibody (OTI1C7) [NBP2-02043]

Western Blot: Mannose Phosphate Isomerase Antibody (OTI1C7) [NBP2-02043]

Western Blot: Mannose Phosphate Isomerase Antibody (1C7) [NBP2-02043] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Mannose Phosphate Isomerase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Mannose Phosphate Isomerase.
Immunocytochemistry/Immunofluorescence: Mannose Phosphate Isomerase Antibody (1C7) [NBP2-02043] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Mannose Phosphate Isomerase.
Immunohistochemistry-Paraffin: Mannose Phosphate Isomerase Antibody (OTI1C7) [NBP2-02043]

Immunohistochemistry-Paraffin: Mannose Phosphate Isomerase Antibody (OTI1C7) [NBP2-02043]

Immunohistochemistry-Paraffin: Mannose Phosphate Isomerase Antibody (1C7) [NBP2-02043] - Staining of paraffin-embedded Human prostate tissue using anti-Mannose Phosphate Isomerase mouse monoclonal antibody.

Applications for Mannose Phosphate Isomerase Antibody (OTI1C7)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Mannose Phosphate Isomerase

Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. [provided by RefSeq].

Alternate Names

CDG1B, EC 5.3.1, EC 5.3.1.8, FLJ39201, mannose phosphate isomerase, mannose-6- phosphate isomerase, mannose-6-phosphate isomerase, Phosphohexomutase, Phosphomannose isomerase, phosphomannose isomerase 1, PMI, PMI1phosphohexomutase

Entrez Gene IDs

4351 (Human)

Gene Symbol

MPI

Additional Mannose Phosphate Isomerase Products

Product Documents for Mannose Phosphate Isomerase Antibody (OTI1C7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mannose Phosphate Isomerase Antibody (OTI1C7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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