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MAP1 Antibody (4A1)

Novus Biologicals, part of Bio-Techne | Catalog # H00064112-M02

Novus Biologicals, part of Bio-Techne
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H00064112-M02

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4A1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

MOAP1 (AAH15044.1, 1 a.a. ~ 351 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTLRLLEDWCRGMDMNPRKALLIAGISQSCSVAEIEEALQAGLAPLGEYRLLGRMFRRDENRKVALVGLTAETSHALVPKEIPGKGGIWRVIFKPPDPDNTFLSRLNEFLAGEGMTVGELSRALGHENGSLDPEQGMIPEMWAPMLAQALEALQPALQCLKYKKLRVFSGRESPEPGEEEFGRWMFHTTQMIKAWQVPDVEKRRRLLESLRGPALDVIRVLKINNPLITVDECLQALEEVFGVTDNPRELQVKYLTTYQKDEEKLSAYVLRLEPLLQKLVQRGAIERDAVNQARLDQVIAGAVHKTIRRELNLPEDGPAPGFLQLLVLIKDYEAAEEEEALLQAILEGNFT

Reactivity Notes

This product is reactive against Human.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for MAP1 Antibody (4A1)

Western Blot: MAP1 Antibody (4A1) [H00064112-M02]

Western Blot: MAP1 Antibody (4A1) [H00064112-M02]

Western Blot: MAP1 Antibody (4A1) [H00064112-M02] - Analysis of MOAP1 expression in transfected 293T cell line by MOAP1 monoclonal antibody (M02), clone 4A1.Lane 1: MOAP1 transfected lysate(39.5 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: MAP1 Antibody (4A1) [H00064112-M02]

Immunocytochemistry/ Immunofluorescence: MAP1 Antibody (4A1) [H00064112-M02]

Immunocytochemistry/Immunofluorescence: MAP1 Antibody (4A1) [H00064112-M02] - Analysis of monoclonal antibody to MOAP1 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: MAP1 Antibody (4A1) [H00064112-M02] - Detection limit for recombinant GST tagged MOAP1 is 0.3 ng/ml as a capture antibody.

Applications for MAP1 Antibody (4A1)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is useful for ELISA, Western Blot
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MAP1

A protein termed MAP1 has recently been identified. This protein contains a BH3-like domain and induces caspase-dependent apoptosis in mammalian cells when overexpressed. It forms homodimers and associates with Bcl-2 family members such as Bax, Bcl-2, and Bcl-XL in vitro and in vivo. It has been suggested that MAP1 associates with the tumor suppressor RASSF1A following death receptor activation, allowing a conformational change in Bax that leads to cellular apoptosis.

Alternate Names

MAP1, MAP-1PNMA4Paraneoplastic antigen Ma4, modulator of apoptosis 1, paraneoplastic antigen like 4

Gene Symbol

MOAP1

Additional MAP1 Products

Product Documents for MAP1 Antibody (4A1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MAP1 Antibody (4A1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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