MAP2 Antibody (CL5420)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61417

Novus Biologicals, part of Bio-Techne
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NBP2-61417
NBP2-61417-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Single Cell Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL5420

Concentration

1 mg/ml

View all MAP2 Antibodies »

Product Specifications

Immunogen

MAP2 Antibody (CL5420) was developed against a human recombinant protein corresponding to amino acids: EAKAPHWTSAPLTEASAHSHPPEIKDQGGAGEGLVRSANGFPYREDEEGAFGEHGSQGTYSNTKENGINGELTSADRETAEEVSARIVQVVTAEAVAVLKGEQEKEAQHKDQTAALPLAAEETANLPPSPPPSPASEQTVT

Epitope

PPEIKDQGGAGEGLV

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Cytoskeleton

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

199 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAP2 Antibody (CL5420)

Western Blot: MAP2 Antibody (CL5420) [NBP2-61417]

Western Blot: MAP2 Antibody (CL5420) [NBP2-61417]

Western Blot: MAP2 Antibody (CL5420) [NBP2-61417] - Analysis in human cell line SH-SY5Y. Observed molecular weight 89 and 280 kDA.
Immunocytochemistry/ Immunofluorescence: MAP2 Antibody (CL5420) [NBP2-61417]

Immunocytochemistry/ Immunofluorescence: MAP2 Antibody (CL5420) [NBP2-61417]

Immunocytochemistry/Immunofluorescence: MAP2 Antibody (CL5420) [NBP2-61417] - Staining of SH-SY5Y cells using the Anti-MAP2 monoclonal antibody, showing specific staining in the cytosol and in microtubules in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MAP2 Antibody (CL5420) [NBP2-61417]

Immunohistochemistry-Paraffin: MAP2 Antibody (CL5420) [NBP2-61417]

Immunohistochemistry-Paraffin: MAP2 Antibody (CL5420) [NBP2-61417] - Staining of human tonsil shows absence of positivity in lymphoid cells as expected (negative control).
View 3 more images

Applications for MAP2 Antibody (CL5420)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Single Cell Western

100 ug/ml

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAP2

Microtubule-associated protein 2 also known as MAP2 (theoretical molecular weight 280 kDa) belongs to the MAP2/Tau family which in addition to the two neuronal forms MAP2 and Tau also includes MAP4 (1). For MAP2, four different isoforms have been identified which are produced through alternative splicing including MAP2a, MAP2b, MAP2c and MAP2d (1). MAP2 isoforms contain multiple microtubule-binding domains located near the carboxy terminal, a variably sized amino-terminal projection domain, and a conserved protein kinase A binding domain. MAP2c and MAP2d isoforms have a shorter amino-terminal projection domain, lower molecular weight and are identifiable at ~70 kDa in SDS-PAGE analysis (2,3). MAP2's carboxy terminal domain supports interactions with microtubules, actin, and intermediate filaments (2).

MAP2 isoforms are developmentally regulated and differentially expressed in neurons and some glia. MAP2c is predominantly expressed in the developing brain while the other isoforms are expressed in the adult brain. The distribution of MAP2 isoforms also varies, with MAP2a and MAP2b predominantly localized to dendrites, while MAP2c is also found in axons. Lastly, the expression of MAP2d is not limited to neurons and may be found in glia, specifically oligodendrocytes (1, 2). MAP2 isoforms associate with microtubules and mediate their interaction with actin filaments thereby playing a critical role in organizing the microtubule-actin network. In neurons, MAP2 isoforms are implicated in different processes including neurite initiation, elongation and stabilization as well as axon and dendrite formation (2). Knockout of MAP expression in animal models results in a variety of functional and structural brain defects according to the isoform affected (e.g., reduced LTP and LTD, reduced myelination, absence of corpus collosum, motor system malfunction, abnormal hippocampal dendritic morphology, abnormal synaptic plasticity) (4).

References

1. Dehmelt, L., & Halpain, S. (2005). The MAP2/Tau family of microtubule-associated proteins. Genome Biology. https://doi.org/10.1186/gb-2004-6-1-204

2. Mohan, R., & John, A. (2015). Microtubule-associated proteins as direct crosslinkers of actin filaments and microtubules. IUBMB Life. https://doi.org/10.1002/iub.1384

3. Shafit-Zagardo, B., & Kalcheva, N. (1998). Making sense of the multiple MAP-2 transcripts and their role in the neuron. Molecular Neurobiology. https://doi.org/10.1007/BF02740642

4. Tortosa, E., Kapitein, L. C., & Hoogenraad, C. C. (2016). Microtubule organization and microtubule-associated proteins (MAPs). In Dendrites: Development and Disease. https://doi.org/10.1007/978-4-431-56050-0_3

Long Name

Microtubule-associated Protein 2

Alternate Names

DKFZp686I2148, MAP-2, MAP2A, MAP2B, MAP2C, Microtubule Associated Protein 2, microtubule-associated protein 2, MTAP2

Gene Symbol

MAP2

Additional MAP2 Products

Product Documents for MAP2 Antibody (CL5420)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MAP2 Antibody (CL5420)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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