MAP3K13 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81272
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KKYPGTYKRHPVRPIIHPNAMEKLMKRKGVPHKSGMQTKRPDLLRSEGIPTTEVAPTASPLSGSPKMSTSSSKSRYRSKPRHRRGNSRGSHSDFAAILKNQPAQENSPHPTYLHQAQSQYPSLHHHNSLQQQYQQP
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (83%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MAP3K13 Antibody
Western Blot: MAP3K13 Antibody [NBP1-81272]
Western Blot: MAP3K13 Antibody [NBP1-81272] - Analysis in human cell line SK-BR-3.Immunocytochemistry/ Immunofluorescence: MAP3K13 Antibody [NBP1-81272]
Immunocytochemistry/Immunofluorescence: MAP3K13 Antibody [NBP1-81272] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & microtubule organizing center. Antibody staining is shown in green.Immunohistochemistry-Paraffin: MAP3K13 Antibody [NBP1-81272]
Immunohistochemistry-Paraffin: MAP3K13 Antibody [NBP1-81272] - Staining of human placenta shows strong membranous positivity in trphoblastic cells.Applications for MAP3K13 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. Simple Western reported by internal validation: Tested in mouse retinal ganglion cells; separated by size.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MAP3K13
Long Name
Mitogen-activated protein kinase kinase kinase 13
Alternate Names
EC 2.7.11.25, LZK, MLK
Gene Symbol
MAP3K13
Additional MAP3K13 Products
Product Documents for MAP3K13 Antibody
Product Specific Notices for MAP3K13 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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