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MAP3K13 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81272

Novus Biologicals, part of Bio-Techne
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NBP1-81272
NBP1-81272-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KKYPGTYKRHPVRPIIHPNAMEKLMKRKGVPHKSGMQTKRPDLLRSEGIPTTEVAPTASPLSGSPKMSTSSSKSRYRSKPRHRRGNSRGSHSDFAAILKNQPAQENSPHPTYLHQAQSQYPSLHHHNSLQQQYQQP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (83%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAP3K13 Antibody

Western Blot: MAP3K13 Antibody [NBP1-81272]

Western Blot: MAP3K13 Antibody [NBP1-81272]

Western Blot: MAP3K13 Antibody [NBP1-81272] - Analysis in human cell line SK-BR-3.
Immunocytochemistry/ Immunofluorescence: MAP3K13 Antibody [NBP1-81272]

Immunocytochemistry/ Immunofluorescence: MAP3K13 Antibody [NBP1-81272]

Immunocytochemistry/Immunofluorescence: MAP3K13 Antibody [NBP1-81272] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & microtubule organizing center. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MAP3K13 Antibody [NBP1-81272]

Immunohistochemistry-Paraffin: MAP3K13 Antibody [NBP1-81272]

Immunohistochemistry-Paraffin: MAP3K13 Antibody [NBP1-81272] - Staining of human placenta shows strong membranous positivity in trphoblastic cells.

Applications for MAP3K13 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. Simple Western reported by internal validation: Tested in mouse retinal ganglion cells; separated by size.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAP3K13

MAP3K13 is encoded by this gene is a member of serine/threonine protein kinase family. This kinase contains a dual leucine-zipper motif, and has been shown to form dimers/oligomers through its leucine-zipper motif. This kinase can phosphorylate and activate MAPK8/JNK, MAP2K7/MKK7, which suggests a role in the JNK signaling pathway. [provided by RefSeq]

Long Name

Mitogen-activated protein kinase kinase kinase 13

Alternate Names

EC 2.7.11.25, LZK, MLK

Gene Symbol

MAP3K13

Additional MAP3K13 Products

Product Documents for MAP3K13 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MAP3K13 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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