MAP4K5 Antibody (3H3)
Novus Biologicals, part of Bio-Techne | Catalog # H00011183-M01
![ELISA: MAP4K5 Antibody (3H3) [H00011183-M01]](https://resources.bio-techne.com/images/products/MAP4K5-Antibody-3H3-ELISA-H00011183-M01-img0010.jpg "ELISA: MAP4K5 Antibody (3H3) [H00011183-M01]")
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3H3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
MAP4K5 (AAH36013, 361 a.a. ~ 470 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SSDPNFMLQWNPFVDGANTGKSTSKRAIPPPLPPKPRISSYPEDNFPDEEKASTIKHCPDSESRAPQILRRQSSPSCGPVAETSSIGNGDGISKLMSENTEGSAQAPQLP
Specificity
MAP4K5 - mitogen-activated protein kinase kinase kinase kinase 5
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for MAP4K5 Antibody (3H3)
![Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01]](https://resources.bio-techne.com/images/products/MAP4K5-Antibody-3H3-Western-Blot-H00011183-M01-img0009.jpg "Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01]")
Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01]
Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01] - Analysis of MAP4K5 expression in transfected 293T cell line by MAP4K5 monoclonal antibody (M01), clone 3H3.Lane 1: MAP4K5 transfected lysate(95.04 KDa).Lane 2: Non-transfected lysate.![Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01]](https://resources.bio-techne.com/images/products/MAP4K5-Antibody-3H3-Western-Blot-H00011183-M01-img0001.jpg "Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01]")
Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01]
Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01] - Western Blot analysis of MAP4K5 expression in 293 ( Cat # L026V1 ).![Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01]](https://resources.bio-techne.com/images/products/MAP4K5-Antibody-3H3-Western-Blot-H00011183-M01-img0007.jpg "Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01]")
Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01]
Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01] - Analysis of MAP4K5 over-expressed 293 cell line, cotransfected with MAP4K5 Validated Chimera RNAi ( Cat # H00011183-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MAP4K5 monoclonal antibody (M01) clone 3H3 (Cat # H00011183-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Applications for MAP4K5 Antibody (3H3)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: MAP4K5
Long Name
Hepatocyte Pogenitor Kinase-like/Germinal Center Knase-like Kinase
Alternate Names
GCKR, KHS1, MAPKKKK5
Entrez Gene IDs
11183 (Human)
Gene Symbol
MAP4K5
OMIM
604923 (Human)
UniProt
Additional MAP4K5 Products
Product Documents for MAP4K5 Antibody (3H3)
Product Specific Notices for MAP4K5 Antibody (3H3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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